The London School of Hygiene & Tropical Medicine, WC1E 7HT, London, UK.
Future Microbiol. 2020 Mar;15:241-257. doi: 10.2217/fmb-2019-0165.
We sought to characterize the contribution of the -OTase, PglL, to virulence in two spp. by comparing isogenic mutants in with the related species, . We utilized an array of assays in addition to and murine models to assess virulence of the mutant and wild-type strains in each species. We found that contributes to biofilm and twitching motility in both species. uniquely affected morphology; cell invasion; intracellular motility; plaque formation and intergenus competition in . This mutant was attenuated in the murine model, and extended survival in a vaccine-challenge experiment. Our data support a broad role for in bacterial fitness and virulence, particularly in .
我们试图通过比较与相关物种 具有相同遗传背景的 种的突变株，来描绘 -OTase（PglL）对两种 种毒力的贡献。我们利用了一系列的 检测方法，以及 和鼠类的 模型，来评估每种 种野生型和突变株的毒力。我们发现 在两种 种中均有助于生物膜和翻滚运动。 独特地影响形态；细胞侵袭；细胞内运动；噬菌斑形成和种间竞争。该突变株在鼠类模型中减毒，并且在疫苗挑战实验中延长了存活时间。我们的数据支持 在细菌适应性和毒力中发挥广泛作用，特别是在 中。
