State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Nano Lett. 2020 May 13;20(5):3602-3610. doi: 10.1021/acs.nanolett.0c00529. Epub 2020 Apr 14.
Antimicrobial peptides (AMPs) have been an attractive alternative to traditional antibiotics. However, considerable efforts are needed to further enhance their antimicrobial effects and stability against bacterial degradation. Tetrahedral framework nucleic acids (tFNAs), a new class of three-dimensional nanostructures, have been utilized as a delivery vehicle. In this study, tFNAs were combined for the first time with an antimicrobial peptide GL13K, and the effects of the resultant complexes against (sensitive to GL13K) and (capable of degrading GL13K) were investigated. tFNA-based delivery enhanced the effects of GL13K against . The tFNA vehicle both increased bacterial uptake and promoted membrane destabilization. Moreover, it enhanced the effects of GL13K against by protecting the peptide against degradation in the protease-rich extracellular environment. Therefore, tFNA provides a delivery vehicle for AMPs targeting a broad range of disease.
抗菌肽 (AMPs) 一直是传统抗生素的一种有吸引力的替代品。然而，需要付出相当大的努力来进一步提高它们的抗菌效果和稳定性，以抵抗细菌的降解。四面体框架核酸 (tFNA)，一种新型的三维纳米结构，已被用作一种递送载体。在这项研究中，tFNA 首次与抗菌肽 GL13K 结合，研究了所得复合物对 （对 GL13K 敏感）和 （能够降解 GL13K）的作用。基于 tFNA 的递药增强了 GL13K 对 的作用。tFNA 载体既增加了细菌摄取，又促进了膜的去稳定性。此外，它通过在富含蛋白酶的细胞外环境中保护肽免受降解，增强了 GL13K 对 的作用。因此，tFNA 为针对广泛疾病的 AMP 提供了一种递送载体。
