Leibniz Institute for Farm Animal Biology, Institute of Muscle Biology and Growth, 18196, Dummerstorf, Germany.
Freie Universität Berlin, Institute of Veterinary Physiology, 14163, Berlin, Germany.
Sci Rep. 2020 Apr 9;10(1):6149. doi: 10.1038/s41598-020-62779-1.
Low birth weight (LBW) can cause lifelong impairments in muscle development and growth. Satellite cells (SC) and their progeny are crucial contributors to myogenic processes. This study provides new data on LBW in piglets combining insights on energy metabolism, muscle capillarization and differences in SC presence and function. To this aim, muscle tissues as well as isolated myogenic cells of 4-day-old German Landrace piglets were analyzed. For the first time two heterogeneous SC subpopulations, which contribute differently to muscle development, were isolated from LBW pigs by Percoll density gradient centrifugation. The muscles of LBW piglets showed a reduced DNA, RNA, and protein content as well as lower activity of the muscle specific enzymes CK, ICDH, and LDH compared to their normal birth weight siblings. We assume that deficits in energy metabolism and capillarization are associated with reduced bioavailability of SC, possibly leading to early exhaustion of the SC reserve cell pool and the cells' premature differentiation.
低出生体重(LBW)可导致肌肉发育和生长的终身损伤。卫星细胞(SC)及其后代是肌肉发生过程中的重要贡献者。本研究通过能量代谢、肌肉毛细血管化以及 SC 存在和功能的差异,为仔猪 LBW 提供了新的数据。为此,分析了 4 日龄德国长白仔猪的肌肉组织和分离的肌原细胞。首次通过 Percoll 密度梯度离心法从 LBW 仔猪中分离出两种不同的具有异质性的 SC 亚群,它们对肌肉发育的贡献不同。与正常出生体重的兄弟姐妹相比,LBW 仔猪的肌肉 DNA、RNA 和蛋白质含量较低,肌肉特异性酶 CK、ICDH 和 LDH 的活性也较低。我们假设能量代谢和毛细血管化的缺陷与 SC 的生物利用度降低有关,这可能导致 SC 储备细胞池的早期耗尽和细胞的过早分化。