Long Non-Coding RNA Sponges microRNA-532 and Promotes Oncogenicity of Clear Cell Renal Cell Carcinoma by Increasing ETS1 Expression.

PURPOSE

The long non-coding RNA cancer susceptibility 19 () is recognized as an important regulator in gastric cancer, colorectal cancer, and non-small cell lung cancer. Nevertheless, to the best of our knowledge, the expression status and detailed roles of in clear cell renal cell carcinoma (ccRCC) have not been elucidated. Hence, we aimed to determine expression in ccRCC and investigate its roles in ccRCC oncogenicity. The molecular mechanisms underlying functions in ccRCC were also determined.

METHODS

expression was measured by using reverse transcription-quantitative polymerase chain reaction. The effects of on ccRCC cell proliferation, colony formation, migration, and invasiveness in vitro, as well as on tumor growth in vivo, were examined by the MTT assay, colony formation assay, cell migration and invasiveness assays, and tumor xenograft in nude nice, respectively.

RESULTS

was overexpressed in ccRCC tissues and cell lines. High expression of was closely associated with unfavorable clinicopathological parameters and predicted negative clinical outcomes in patients with ccRCC. Knockdown of decreased ccRCC cell proliferation, colony formation, migration, and invasiveness, as well as attenuated tumor growth in vivo. Mechanistically, functioned as a competing endogenous RNA and upregulated the expression of ETS proto-oncogene 1 (ETS1) through sponging microRNA-532 (miR-532). Furthermore, rescue assays revealed that inhibiting miR-532 or restoring ETS1 expression partially abolished the impacts of knockdown on ccRCC cells.

CONCLUSION

The CASC19/miR-532/ETS1 regulatory pathway is crucial for the malignant manifestations of ccRCC, which makes it an attractive target for potential treatments of ccRCC.