Tayh Ghassan, Nagarjuna Daram, Sallem Rym Ben, Verma Vivek, Chairat Sarra, Boudabous Abdellatif, Yadav Manisha, Slama Karim Ben
PhD, Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis-El Manar, 2092 Tunis, Tunisie.
PhD, Dr. B. R. Ambedkar Centre for Biomedical Research (ACBR), University of Delhi, Delhi-110007, India.
Germs. 2020 Mar 2;10(1):18-26. doi: 10.18683/germs.2020.1181. eCollection 2020 Mar.
Even though the increasing incidence of VIM-producing and has been reported worldwide, studies are still lacking in Palestine. The aim of this study was to screen carbapenem-resistant and bacteria in the Gaza Strip, Palestine and further to characterize carbapenemase-producing isolates.
A total of 69 and 27 isolates were obtained from three Gaza hospitals and recovered from urine, wound swabs, blood and ear discharge. The screening for metallo-β-lactamases (MBLs) was performed by using the imipenem-EDTA disc synergy test. The detection of β-lactamases genes, detection of non-β-lactam genes and the characterization of integrons were performed by PCR and sequencing. The clonal relationship among the isolates was determined by pulsed-field gel electrophoresis (PFGE).
Our study showed that 4 (5.8%) and 5 (18.5%) were positive by the imipenem-EDTA disc synergy test. was detected in six isolates and was identified in three isolates. The β-lactamases genes in the VIM-producing isolates were (n=3), (n=1), (n=3), (n=1), (n=1) and (n=1). -Ib-cr gene was confirmed in four and in two isolates. was identified in two isolates. The class 1 integron was identified with the different gene cassette; and were identified.
Our study indicated for the first time the emergence of multidrug-resistant VIM-containing and isolates of clinical origin in Gaza Strip hospitals.
尽管产VIM的[细菌名称]的发病率在全球范围内呈上升趋势，但巴勒斯坦仍缺乏相关研究。本研究的目的是筛查巴勒斯坦加沙地带耐碳青霉烯类[细菌名称]和[细菌名称]，并进一步鉴定产碳青霉烯酶的分离株。
从加沙的三家医院共获得69株[细菌名称]和27株[细菌名称]分离株，分别从尿液、伤口拭子、血液和耳分泌物中分离得到。采用亚胺培南-EDTA纸片协同试验筛选金属β-内酰胺酶（MBLs）。通过PCR和测序检测β-内酰胺酶基因、非β-内酰胺基因并鉴定整合子。通过脉冲场凝胶电泳（PFGE）确定分离株之间的克隆关系。
我们的研究表明，亚胺培南-EDTA纸片协同试验中4株[细菌名称]（5.8%）和5株[细菌名称]（18.5%）呈阳性。在6株分离株中检测到[酶名称]，在3株分离株中鉴定出[酶名称]。产VIM的[细菌名称]分离株中的β-内酰胺酶基因分别为[基因名称1]（n = 3）、[基因名称2]（n = 1）、[基因名称3]（n = 3）、[基因名称4]（n = 1）、[基因名称5]（n = 1）和[基因名称6]（n = 1）。在4株[细菌名称]和2株[细菌名称]分离株中证实了-Ib-cr基因。在2株[细菌名称]分离株中鉴定出[基因名称]。1类整合子通过不同的基因盒鉴定；鉴定出[基因盒名称1]和[基因盒名称2]。
我们的研究首次表明加沙地带医院出现了临床来源的含VIM的多重耐药[细菌名称]和[细菌名称]分离株。
