Molecular Characterization of Multidrug Resistant Uropathogenic Isolates from Jordanian Patients.

BACKGROUND

Emergence of multi-drug resistant uropathogenic strains is an increasing problem to empirical treatment of urinary tract infections in many countries. This study investigated the magnitude of this problem in Jordan.

METHODS

A total of 262 isolates were recovered from urine samples of Jordanian patients which were suspected to have urinary tract infections (UTIs). All isolates were primarily identified by routine biochemical tests and tested for antimicrobial susceptibility by disc diffusion method. Fifty representative Multidrug Resistance (MDR) isolates to 3 or more antibiotic classes were tested for the presence of resistance genes of 1, 9 and 15, carbapenemase (), fluoroquinolones mutated genes () and clone of ST131 type using PCR methods.

RESULTS

A total of 150/262 (57.3%) of isolates were MDR. Urine samples of hospitalized patients showed significantly more MDR isolates than outpatients. Fifty representative MDR isolates indicated the following molecular characteristics: All were positive for mutated gene and and for ST131 clone, and 78% were positive for genes of , 76% for and for 8% , respectively. Additionally, all 50 MDR isolates were negative for carbapenemase genes (), except of one isolate was positive for

CONCLUSION

This study indicates alarming high rates recovery of MDR uropathogenic from Jordanian patients associated with high rates of positive ST131 clone, fluoroquinolone resistant and important types of blaCTX-M.