Mortimer A J, Dyson M
National Research Council of Canada, Ottawa.
Ultrasound Med Biol. 1988;14(6):499-506. doi: 10.1016/0301-5629(88)90111-1.
The effect of therapeutic ultrasound on calcium uptake by embryonic chick 3T3 fibroblasts has been studied using calcium-45 radiotracer techniques. Cells were treated while suspended in culture medium at 36 degrees C at intensities from 0.25 W/cm2 SPPA to 1.5 W/cm2 SPPA (1 MHz, pulsed 2 ms on:8 ms off) and for exposure times from 1 min to 20 min. Ultrasound treatment was found to increase calcium uptake for SPPA intensities from 0.5 to 1.0 W/cm2 SPPA, with a maximum increase of 18% after a 5 min exposure. Calcium uptake also increased with increasing exposure time. Measurements performed up to 20 min after treatment showed that the cell was able to reduce this calcium influx, indicating that the membrane did not suffer irreparable damage as a result of the ultrasound exposure.
利用钙 - 45放射性示踪技术研究了治疗性超声对胚胎鸡3T3成纤维细胞钙摄取的影响。细胞悬浮于培养基中，在36摄氏度下，以0.25瓦/平方厘米声功率峰值平均声强（SPPA）至1.5瓦/平方厘米SPPA（1兆赫，脉冲占空比为2毫秒开启：8毫秒关闭）的强度处理1分钟至20分钟。研究发现，对于0.5至1.0瓦/平方厘米SPPA的声功率峰值平均声强，超声处理会增加钙摄取，在暴露5分钟后最大增加18%。钙摄取也随暴露时间的增加而增加。在处理后长达20分钟进行的测量表明，细胞能够减少这种钙内流，这表明细胞膜并未因超声暴露而遭受不可修复的损伤。
