Department of Periodontology and Oral Surgery, Faculty of Medicine, 4000 Liège, Belgium.
Department of Life Science and Medicine (DLSM), University of Luxembourg, 4365 Esch/Alzette, Luxembourg.
Int J Mol Sci. 2022 Oct 17;23(20):12426. doi: 10.3390/ijms232012426.
The present study evaluated the influence of Low-Intensity Pulsed Ultrasound (LIPUS) on the regeneration processes of non-critical-size bone defects in irradiated and non-irradiated rabbit tibias. Bone defects were surgically created on both tibiae of six rabbits. The control group had no additional treatment. In one intervention group, one tibia was irradiated with 15 Gy in a single dose. A second group was treated with LIPUS, and a third with a combination of both treatments. The control samples showed 83.10% ± 17.79% of bone repair after 9 weeks, while the irradiated bone had regenerated significantly less during the same period (66.42% ± 29.36%). The LIPUS treatment on irradiated bones performed a 79.21% ± 21.07% bone fill and could not significantly improve the response compared to the non-treated irradiated specimens. However, LIPUS treatment on non-irradiated bone showed bone formations beyond the size defect (115.91% ± 33.69%), which was a highly significant increase when compared to the control group or any irradiated group. The application of ultrasound to healthy bone produced highly significant and enhanced bone formations with 36.70% more regenerated bone when compared to the same application on irradiated bone. LIPUS vibration stimuli may be considered as a promising complementary treatment approach in non-irradiated bone regeneration procedures to shorten the treatment and enhance bone healing. In irradiated bones, the effect of ultrasound application is less clear, and further studies are needed to refine the dynamics of the present results.
本研究评估了低强度脉冲超声（LIPUS）对辐照和未辐照兔胫骨非临界尺寸骨缺损再生过程的影响。在六只兔子的双侧胫骨上手术造成骨缺损。对照组没有额外的治疗。在一组干预组中，一只胫骨接受 15 Gy 的单次剂量照射。第二组接受 LIPUS 治疗，第三组同时接受两种治疗。对照样本在 9 周后显示 83.10%±17.79%的骨修复，而同一时期辐照骨的再生明显较少（66.42%±29.36%）。LIPUS 治疗辐照骨可实现 79.21%±21.07%的骨填充，与未治疗的辐照标本相比，其反应不能显著改善。然而，LIPUS 治疗未辐照骨可使骨形成超出缺损大小（115.91%±33.69%），与对照组或任何辐照组相比，这是一个高度显著的增加。与相同的辐照骨应用相比，将超声应用于健康骨可产生高度显著的和增强的骨形成，再生骨增加 36.70%。LIPUS 振动刺激可被视为非辐照骨再生过程中一种有前途的补充治疗方法，可缩短治疗时间并增强骨愈合。在辐照骨中，超声应用的效果不太明确，需要进一步研究以细化当前结果的动力学。
