Suppiger Stephanie, Astasov-Frauenhoffer Monika, Schweizer Irene, Waltimo Tuomas, Kulik Eva M
Department of Oral Health & Medicine, University Center for Dental Medicine, University of Basel, 4058 Basel, Switzerland.
Department Research, University Center for Dental Medicine, University of Basel, 4058 Basel, Switzerland.
Antibiotics (Basel). 2020 Apr 8;9(4):167. doi: 10.3390/antibiotics9040167.
The aim of this study was to analyze the potential influence of long-term exposure in subinhibitory concentrations of chlorhexidine on the emergence of tolerant and/or persistent cells in oral streptococci. The two oral streptococcal isolates ATCC25175 and ATCC33402 were incubated, after long-term subinhibitory exposure to chlorhexidine, in liquid growth media containing high concentrations of chlorhexidine. A distinct subpopulation of more chlorhexidine-tolerant cells could be detected in streptococci that had been previously exposed to subinhibitory concentrations of chlorhexidine but not in the control strains. These more biocide-tolerant and persisting microbial subpopulations might also arise in vivo. Therefore, the rational and proper use of antimicrobials in dentistry, especially when used over a long period of time, is crucial.
本研究的目的是分析长期暴露于亚抑菌浓度的洗必泰对口腔链球菌中耐受性和/或持续性细胞出现的潜在影响。两种口腔链球菌分离株ATCC25175和ATCC33402在长期亚抑菌暴露于洗必泰后，在含有高浓度洗必泰的液体生长培养基中培养。在先前暴露于亚抑菌浓度洗必泰的链球菌中可检测到一个明显的更耐洗必泰细胞亚群，而在对照菌株中则未检测到。这些更耐杀菌剂且持续存在的微生物亚群也可能在体内出现。因此，在牙科中合理、正确地使用抗菌药物，尤其是长期使用时，至关重要。
