Paradiso Vito Michele, Castellino Maria, Renna Massimiliano, Santamaria Pietro, Caponio Francesco
Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy.
Institute of Sciences of Food Production, National Research Council of Italy, Via Amendola 122/O, 70126 Bari, Italy.
Foods. 2020 Apr 8;9(4):459. doi: 10.3390/foods9040459.
Microgreens are gaining increasing interest as a potential functional food due to their relevant contents of micronutrients and bioactive compounds, including carotenoids. Nevertheless, the analysis of carotenoids is inherently difficult, due to their thermal and chemical susceptibility, as well as to their varying polarity. From this point of view, extraction is the most critical step, compared to chromatographic separation and detection. Thus, the reliability of data on carotenoids should be guaranteed by a constant focus on analytical issues, with appropriate adaptations to each sample matrix. In this research, a specific extraction procedure for the analysis of carotenoids in microgreens was developed. Solvent composition, extraction time, solvent/sample ratio, and repeated extractions were evaluated. The obtained protocol showed recovery of 97.2%, limits of quantitation of 5.2 μg·g for lutein and 15.9 μg·g for β-carotene, as well as intra-day mean repeatability of 5.7% and inter-day mean repeatability of 4.7%.
由于含有包括类胡萝卜素在内的微量营养素和生物活性化合物，微型蔬菜作为一种潜在的功能性食品正越来越受到关注。然而，由于类胡萝卜素对热和化学的敏感性以及其极性的变化，对其进行分析本质上具有难度。从这一角度来看，与色谱分离和检测相比，提取是最关键的步骤。因此，应始终关注分析问题，并针对每个样品基质进行适当调整，以确保类胡萝卜素数据的可靠性。在本研究中，开发了一种用于分析微型蔬菜中类胡萝卜素的特定提取程序。对溶剂组成、提取时间、溶剂/样品比例和重复提取进行了评估。所获得的方案显示回收率为97.2%，叶黄素的定量限为5.2μg·g，β-胡萝卜素的定量限为15.9μg·g，日内平均重复性为5.7%，日间平均重复性为4.7%。
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