Phytochemistry and Phytopharmacology Division, KSCSTE - Jawaharlal Nehru Tropical Botanic Garden and Research Institute (Research Centre, University of Kerala), Palode, Thiruvananthapuram, 695 562 Kerala, India; University of Kerala, Thiruvananthapuram, 695 034 Kerala, India.
Department of Zoology, University of Kerala, Thiruvananthapuram, 695 034 Kerala, India.
Fitoterapia. 2020 Jun;143:104592. doi: 10.1016/j.fitote.2020.104592. Epub 2020 Apr 8.
Two new caged xanthones, wightiic acid (1) 16-O-methyl wightiic acid (2), along with eight known compounds, gaudichaudic acid E (3), isogaudichaudic acid E (4), ursolic acid (5) stigmasterol (6), lupeol (7), glutinol (8), lupenone (9) and stigmasteryl linoleate (10) were isolated from Garcinia wightii T. Anderson. The structures of the compounds were elucidated by spectroscopic means, including 1D and 2D NMR, HR-ESIMS, and the absolute configuration of the new compounds 1 and 2 were determined by analysis of ECD data. Anti-proliferation activities of the four caged xanthones (1-4) were evaluated by MTT assay on MCF-7 and SKBR-3 human breast cancer cells and A-375 human melanoma cells by MTT assay. All the tested compounds exhibited dose dependent antiproliferative activity. Wightiic acid (1) showed remarkable activity with IC value of 4.7 μM and 5.2 μM respectively in A-375 and MCF-7 cells. The compound isogaudichaudic acid E (4) induced potent antiproliferation against SKBR-3 cells with an IC value of 6.1 μM.
从藤黄属植物中分离得到了两种新的笼状黄烷酮，即 wightiic 酸（1）和 16-O-甲基 wightiic 酸（2），以及 8 种已知化合物，gaudichaudic 酸 E（3）、isogaudichaudic 酸 E（4）、熊果酸（5）、豆甾醇（6）、羽扇豆醇（7）、谷甾醇（8）、羽扇烯酮（9）和豆甾醇亚油酸酯（10）。通过光谱学方法，包括 1D 和 2D NMR、HR-ESIMS，确定了化合物的结构，通过分析 ECD 数据确定了新化合物 1 和 2 的绝对构型。通过 MTT 法对 MCF-7 和 SKBR-3 人乳腺癌细胞和 A-375 人黑色素瘤细胞进行了四笼黄烷酮（1-4）的抗增殖活性评价。所有测试的化合物均表现出剂量依赖性的抗增殖活性。wightiic 酸（1）在 A-375 和 MCF-7 细胞中分别表现出 4.7μM 和 5.2μM 的显著活性。化合物 isogaudichaudic 酸 E（4）对 SKBR-3 细胞表现出强烈的抑制增殖作用，IC 值为 6.1μM。
