Developmental Biology Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur - 603203, Chengalpattu District, Tamil Nadu, India.
Developmental Biology Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur - 603203, Chengalpattu District, Tamil Nadu, India.
J Hazard Mater. 2020 Jul 15;394:122533. doi: 10.1016/j.jhazmat.2020.122533. Epub 2020 Mar 27.
Acrylamide, an environmental pollutant, is known to occur in food substances cooked at high temperatures. Studies on various models indicate acrylamide to cause several physiological conditions such as neuro- and reproductive toxicity, and carcinogenesis. In our study, exposure of Drosophila melanogaster (Oregon K strain) to acrylamide via their diet resulted in a concentration and time-dependent mortality, while the surviving flies exhibited significant locomotor deficits, most likely due to oxidative stress-induced neuronal damage. Also, Drosophila embryos exhibited signs of developmental toxicity as evidenced by the alteration in the migration of border cells and cluster cells during the developmental stages, concomitant to modulation in expression of gurken and oskar genes. Curcumin, a known antioxidant has been widely studied for its neuroprotective effects against acrylamide; however; very few studies focus on thymoquinone for its role against food toxicant. Our research focuses on the toxicity elicited by acrylamide and the ability of the antioxidants: thymoquinone, curcumin and combination of thereof, in reversing the same.
丙烯酰胺，一种环境污染物，已知存在于高温烹饪的食物中。对各种模型的研究表明丙烯酰胺会导致多种生理状况，如神经毒性和生殖毒性以及致癌作用。在我们的研究中，通过饮食使黑腹果蝇（Oregon K 品系）接触丙烯酰胺会导致浓度和时间依赖性死亡率，而幸存的果蝇表现出明显的运动缺陷，这很可能是由于氧化应激诱导的神经元损伤所致。此外，果蝇胚胎表现出发育毒性的迹象，这表现在边界细胞和簇状细胞在发育阶段的迁移发生改变，同时 gurken 和 oskar 基因的表达也发生了调节。姜黄素是一种已知的抗氧化剂，已广泛研究其对丙烯酰胺的神经保护作用；然而，很少有研究关注其对食物毒物的对抗作用。我们的研究重点是丙烯酰胺引起的毒性，以及抗氧化剂：百里醌、姜黄素及其组合逆转这种毒性的能力。
