National University of Science and Technology "MISIS", Leninskiy pr. 4, 119049 Moscow, Russia; N. F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Gamaleya Str. 18, 123098 Moscow, Russia.
National University of Science and Technology "MISIS", Leninskiy pr. 4, 119049 Moscow, Russia.
Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110750. doi: 10.1016/j.msec.2020.110750. Epub 2020 Feb 18.
A promising direction for the replacement of expanded bone defects is the development of bioimplants based on synthetic biocompatible materials impregnated with growth factors that stimulate bone remodeling. Novel biomimetic highly porous ultra-high molecular weight polyethylene (UHMWPE)/40% hydroxyapatite (HA) scaffold for reconstructive surgery with the porosity of 85 ± 1% vol. and a diameter of pores in the range of 50-800 μm was developed. The manufacturing process allowed the formation of trabecular-like architecture without additional solvents and thermo-oxidative degradation. Biomimetic UHMWPE/HA scaffold was biocompatible and provided effective tissue ingrowth on a model of critical-sized cranial defects in mice. The combined use of UHMWPE/HA with Bone Morphogenetic Protein-2 (BMP-2) demonstrated intensive mineralized bone formation as early as 3 weeks after surgery. The addition of erythropoietin (EPO) significantly enhanced angiogenesis in newly formed tissues. The effect of EPO of bacterial origin on bone tissue defect healing was demonstrated for the first time. The developed biomimetic highly porous UHMWPE/HA scaffold can be used separately or in combination with rhBMP-2 and EPO for reconstructive surgery to solve the problems associated with difference between implant architecture and trabecular bone, low osteointegration and bioinertness.
一种有前途的替代扩展骨缺损的方法是开发基于合成生物相容性材料的生物植入物，这些材料浸渍有刺激骨重塑的生长因子。新型仿生超高相对分子质量聚乙烯（UHMWPE）/40%羟基磷灰石（HA）多孔支架，用于修复手术，具有 85±1%体积的孔隙率和 50-800μm 范围内的孔径。制造工艺允许在没有额外溶剂和热氧化降解的情况下形成小梁样结构。仿生 UHMWPE/HA 支架具有生物相容性，并在小鼠临界尺寸颅骨缺损模型中提供了有效的组织向内生长。UHMWPE/HA 与骨形态发生蛋白-2（BMP-2）联合使用，早在手术后 3 周就显示出密集的矿化骨形成。添加促红细胞生成素（EPO）可显著增强新形成组织中的血管生成。细菌来源的 EPO 对骨组织缺损愈合的影响首次得到证实。开发的仿生高多孔 UHMWPE/HA 支架可单独使用或与 rhBMP-2 和 EPO 联合使用，用于修复手术，以解决植入物结构与小梁骨之间的差异、低骨整合和生物惰性等问题。
