Li Jin-Xiao, Yan Qian, Liu Na, Zheng Wen-Jiang, Hu Man, Yu Zhao-Min, Zhou Yu-Dian, Wang Xiong-Wen, Liang Feng-Xia, Chen Rui
Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College Huazhong University of Science and Technology, 430022 Wuhan, China.
Guangzhou University of Chinese Medicine, 510405 Guangzhou, China.
Evid Based Complement Alternat Med. 2020 Mar 23;2020:8475840. doi: 10.1155/2020/8475840. eCollection 2020.
At present, the relationship between autophagosomes and the prognosis of various cancers has become a subject of active investigation. A series of studies have demonstrated the correlation between autophagy microtubule-associated protein light chain 3 (LC-3), Beclin-1, and colorectal cancer (CRC). Since autophagy has dual regulatory roles in tumors, the results of this correlation are also uncertain. Hence, we summarized the relationship between Beclin-1, LC-3, and CRC using systematic reviews and meta-analysis to clarify their prognostic significance in it.
PubMed, EMBASE, Cochrane Library, and Web of Science databases were searched online up to April 1, 2019. The quality of the involving studies was assessed against the Newcastle-Ottawa Scale (NOS). Pooled hazard ratio (HR) and 95% confidence interval (CI) in a fixed or random effects model were used to assess the strength of correlation between Beclin-1, LC-3, and CRC.
A total of 9 articles were collected, involving 2,297 patients. Most literatures scored more than 6 points, suggesting that the quality of our including research was acceptable. Our finding suggested that the expression of Beclin-1 was not associated with overall survival (HR = 0.68, 95% CI (0.31-1.52), =0.351). Nonetheless, LC-3 expression exerted significant impact on OS (HR = 0.51, 95% CI (0.35-0.74), < 0.05). Subgroup analysis exhibited that Beclin-1 expression was associated with OS at TNM stage III (HR = 0.04, 95% CI = 0.02-0.08, < 0.05), surgical treatment (HR = 1.53, 95% CI (1.15-2.02), =0.003), and comprehensive treatment (HR = 0.27 95% CI (0.08-0.92), =0.036), respectively. Similarly, the results showed the increased LC-3 expression in CRC was related to OS in multivariate analyses (HR = 0.44, 95% CI (0.34-0.57), < 0.05), stages (HR = 0.51, 95% CI (0.35-0.74), < 0.05), and comprehensive treatment (HR = 0.44, 95% CI (0.34-0.57), < 0.05).
Autophagy-related proteins of LC-3 might be an important marker of CRC progression. However, since the number of the original studies was limited, more well-designed, large-scale, high-quality studies are warranted to provide more convincing and reliable information.
目前，自噬体与各种癌症预后之间的关系已成为积极研究的课题。一系列研究已经证实了自噬微管相关蛋白轻链3（LC-3）、Beclin-1与结直肠癌（CRC）之间的相关性。由于自噬在肿瘤中具有双重调节作用，这种相关性的结果也不确定。因此，我们通过系统评价和荟萃分析总结了Beclin-1、LC-3与CRC之间的关系，以阐明它们在其中的预后意义。
截至2019年4月1日，在线检索PubMed、EMBASE、Cochrane图书馆和Web of Science数据库。根据纽卡斯尔-渥太华量表（NOS）评估纳入研究的质量。采用固定效应模型或随机效应模型中的合并风险比（HR）和95%置信区间（CI）来评估Beclin-1、LC-3与CRC之间的相关强度。
共收集到9篇文章，涉及2297例患者。大多数文献得分超过6分，表明我们纳入研究的质量是可以接受的。我们的研究结果表明，Beclin-1的表达与总生存期无关（HR = 0.68，95%CI（0.31 - 1.52），P = 0.351）。然而，LC-3表达对总生存期有显著影响（HR = 0.51，95%CI（0.35 - 0.74），P < 0.05）。亚组分析显示，Beclin-1表达分别与TNM III期的总生存期（HR = 0.04，95%CI = 0.02 - 0.08，P < 0.05）、手术治疗（HR = 1.53，95%CI（1.15 - 2.02），P = 0.003）和综合治疗（HR = 0.27，95%CI（0.08 - 0.92），P = 0.036）相关。同样，结果显示在多变量分析中，CRC中LC-3表达的增加与总生存期（HR = 0.44，95%CI（0.34 - 0.57），P < 0.05）、分期（HR = 0.51，95%CI（0.35 - 0.74），P < 0.05）和综合治疗（HR = 0.44，95%CI（0.34 - 0.57），P < 0.05）相关。
LC-3自噬相关蛋白可能是CRC进展的重要标志物。然而，由于原始研究数量有限，需要更多设计良好、大规模、高质量的研究来提供更有说服力和可靠的信息。
