Department of Preventive Healthcare, Zhongshan Hospital of Traditional Chinese Medicine, 3 Kangxin Road, Western District, Zhongshan City, 528400 Guangdong Province, China.
Department of Critical Care Medicine, Zhongshan Hospital of Traditional Chinese Medicine, 3 Kangxin Road, Western District, Zhongshan City, 528400 Guangdong Province, China.
Biomed Res Int. 2020 Mar 21;2020:1635324. doi: 10.1155/2020/1635324. eCollection 2020.
High-risk papillomavirus (HR-HPV) testing combined with cytology improves the detection of cervical lesions and increases length of screening intervals. For a population-based HR-HPV survey, testing automation is in great need. The Cobas 4800 HPV Test System is a fully automated assay that can simultaneously detect HPV16, HPV18, and other 12 pooled HR-HPV genotypes. This system has been employed for HR-HPV screening in a number of countries; however, such application in a large population in China has not been documented. In this study, we employed the Cobas 4800 HPV Test System to detect HR-HPV in cervical cytology specimens collected from a total of 5650 asymptomatic women from a region of South China. We reported the following: (1) the prevalence of the 14 genotypes of HR-HPV was 12.96%; (2) for those with HR-HPV infection, 2.25% were positive for HPV16, 0.50% for HPV18, 9.15% for pooled 12 HPV types, and 1.06% for multiple HPV infection; and (3) there was no significant difference in the HR-HPV prevalence among different age groups. HPV16 and HPV18 have been shown to be the predominant HPV types found in cervical cancer patients in some regions in China, indicating that a fully automated assay like the Cobas 4800 HPV Test System is especially valuable for population-based HR-HPV screening in these regions as this assay can concurrently detect HPV16 and HPV18.
高危型人乳头瘤病毒（HR-HPV）检测联合细胞学检查可提高宫颈病变的检出率，并延长筛查间隔。对于基于人群的 HR-HPV 调查，检测自动化非常必要。Cobas 4800 HPV 检测系统是一种全自动检测方法，可同时检测 HPV16、HPV18 和其他 12 种 HR-HPV 型别。该系统已在多个国家用于 HR-HPV 筛查；然而，在中国的大规模人群中，尚未有此类应用的报道。在这项研究中，我们使用 Cobas 4800 HPV 检测系统检测了来自中国南方一个地区的 5650 名无症状女性的宫颈细胞学标本中的 HR-HPV。我们报告了以下结果：（1）14 种 HR-HPV 型别的流行率为 12.96%；（2）对于 HR-HPV 感染的患者，HPV16 的阳性率为 2.25%，HPV18 的阳性率为 0.50%，12 种 HPV 型别的合并阳性率为 9.15%，多重 HPV 感染的阳性率为 1.06%；（3）不同年龄组之间 HR-HPV 的流行率无显著差异。HPV16 和 HPV18 已被证明是中国某些地区宫颈癌患者中主要的 HPV 型别，这表明像 Cobas 4800 HPV 检测系统这样的全自动检测方法对于这些地区基于人群的 HR-HPV 筛查特别有价值，因为该检测方法可以同时检测 HPV16 和 HPV18。
