波兰女性宫颈样本中的Cobas 4800人乳头瘤病毒检测
Cobas 4800 HPV detection in cervical samples of Polish women.
作者信息
Smolarz Beata, Samulak Dariusz, Szyłło Krzysztof, Makowska Marianna, Romanowicz Hanna, Kojs Zbigniew, Michalska Magdalena M
机构信息
Laboratory of Cancer Genetics, Department of Pathology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
Department of Obstetrics and Gynaecology and Gynaecological Oncology, Regional Hospital in Kalisz, Poland.
出版信息
Contemp Oncol (Pozn). 2019;23(2):92-95. doi: 10.5114/wo.2019.85880. Epub 2019 Jun 13.
INTRODUCTION
Long-term infection with human papillomavirus (HPV) is the cause of cervical cancer and its precursor - cervical intraepithelial neoplasia (CIN). The presence of HPV infection can be presumed in more than 99% of cases of cervical cancer worldwide. The introduction of DNA testing for the presence of HPV has increased the effectiveness of screening programs for the detection of this cancer. This study aimed to analyze the prevalence of high risk HPV DNA (HR HPV) in females from Poland.
MATERIAL AND METHODS
The study was performed on 280 cervical smear samples. In this work we used the Roche Cobas 4800 HPV test to detect the HR HPV in cervical smear samples.
RESULTS
56 patients (20%) proved to be positive regarding HPV-16 DNA and 40 patients (14.28%) regarding HPV-18 DNA. In overall assessment, in 94 patients (33.57%) we detected oncogenic HPV subtypes, other than the two mentioned above. In 90 patients (32.14%) no high risk HPV was detected.
CONCLUSIONS
The Roche Cobas 4800 HPV test is a viable, effective, easy and quick tool in detecting high risk HPV DNA.
引言
人乳头瘤病毒（HPV）的长期感染是宫颈癌及其癌前病变——宫颈上皮内瘤变（CIN）的病因。在全球范围内，超过99%的宫颈癌病例中都可推测存在HPV感染。HPV DNA检测方法的引入提高了这种癌症检测筛查项目的有效性。本研究旨在分析波兰女性中高危型HPV DNA（HR HPV）的流行情况。
材料与方法
本研究对280份宫颈涂片样本进行检测。在这项研究中，我们使用罗氏Cobas 4800 HPV检测法来检测宫颈涂片样本中的HR HPV。
结果
56例患者（20%）的HPV-16 DNA检测呈阳性，40例患者（14.28%）的HPV-18 DNA检测呈阳性。总体评估显示，94例患者（33.57%）检测到除上述两种之外的致癌性HPV亚型。90例患者（32.14%）未检测到高危型HPV。
结论
罗氏Cobas 4800 HPV检测法是检测高危型HPV DNA的一种可行、有效、简便且快速的工具。
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