Research Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Ulmqi, People's Republic of China.
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, People's Republic of China.
Biotechnol Appl Biochem. 2021 Apr;68(2):297-306. doi: 10.1002/bab.1926. Epub 2020 May 22.
A homogeneous monomeric laccase (ASL) from Agaricus sinodeliciosus, with a molecular mass of 65 kDa, was isolated using ion-exchange chromatography (CM-cellulose and Q-Sepharose) and gel-filtration chromatography (Superdex 75). This laccase exhibited maximum activity at 50 °C and pH 5.0. Hg and Cd significantly inhibited its activity. The laccase displayed a K value of 0.9 mM toward 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS). In addition to ABTS, ASL exhibited higher affinity toward o-toluidine and benzidine than other substrates. ASL is able to decolorize malachite green and Eriochrome black T.
从金针菇中分离得到一种均一的单体漆酶（ASL），其相对分子质量为 65 kDa，采用离子交换色谱（CM-纤维素和 Q-琼脂糖）和凝胶过滤色谱（Superdex 75）进行分离。该漆酶在 50°C 和 pH 5.0 时表现出最大活性。Hg 和 Cd 显著抑制其活性。漆酶对 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）的 K 值为 0.9 mM。除 ABTS 外，ASL 对邻甲苯胺和联苯胺的亲和力比对其他底物更高。ASL 能够使孔雀石绿和 Eriochrome 黑 T 脱色。
