Gazwi Hanaa S S, Omar Maha O A, Mahmoud Magda E
Department of Agricultural Chemistry, Faculty of Agriculture, Minia University, El-Minya, Egypt.
Department of Microbiology, Faculty of Agriculture, Minia University, El-Minya, Egypt.
BMC Chem. 2023 Feb 1;17(1):1. doi: 10.1186/s13065-023-00911-8.
Converting seed coat peas (hulls) (SCP) into beneficial products provides a solution for waste treatment. This study aimed to investigate the phytoconstituents and biological activities of SCP extract. Phytochemical screening, total bioactive compounds, and GC-MS analysis were evaluated. Then, the antioxidant, antibacterial, anticancer, and antiviral activities of SCP extract were determined. The results demonstrate that SCP extract has bioactive compounds such as carbohydrates (29.53 ± 4.23 mg/mL), protein (0.24 ± 0.02 mg/mL), phenolics (27.04 ± 0.94 mg GAE/g extract), and flavonoids (17.19 ± 0.48 mg QE/g extract). The existence of more than 16 substances was determined using GC-MS analysis. The extract showed potential antioxidant activities, with the maximum activity seen for extract (IC50 µg/mL) = 79.16 ± 1.77 for DPPH, 67.40 ± 5.20 for ORAC, and 61.22 ± 4.81 for ABTS assays. The SCP extract showed potent antimicrobial activity against four gram-positive bacteria (Bacillus cereus, Streptomyces sp., Staphylococcus aureus, and Salmonella sp.) and two gram-negative bacteria (Escherichia coli and Pseudomonas sp.). SCP extract exhibited potential anticancer activity against lymphoma U937 and leukemic cells (THP1). The extract exhibited potential antiviral activity, with a selectivity index (SI) equal to 11.30 and 18.40 against herpes simplex-II (HSV-2) and adenovirus (Ad7), respectively. The results demonstrate more accurate information about peas by-products' chemical and antioxidant activities in various applications. The chemical components of peas by-products were found to have an in vitro antioxidant, antibacterial, and antiviral activity against leukemia and lymphoma.
将种皮豌豆（外壳）（SCP）转化为有益产品为废物处理提供了一种解决方案。本研究旨在调查SCP提取物的植物成分和生物活性。对其进行了植物化学筛选、总生物活性化合物分析和气相色谱 - 质谱联用（GC - MS）分析。然后，测定了SCP提取物的抗氧化、抗菌、抗癌和抗病毒活性。结果表明，SCP提取物含有生物活性化合物，如碳水化合物（29.53±4.23毫克/毫升）、蛋白质（0.24±0.02毫克/毫升）、酚类（27.04±0.94毫克没食子酸当量/克提取物）和黄酮类（17.19±0.48毫克槲皮素当量/克提取物）。通过GC - MS分析确定存在16种以上的物质。该提取物显示出潜在的抗氧化活性，在二苯基苦味酰基自由基（DPPH）测定中提取物的最大活性为（IC50微克/毫升）= 79.16±1.77，氧自由基吸收能力（ORAC）测定中为67.40±5.20，2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）测定中为61.22±4.81。SCP提取物对四种革兰氏阳性菌（蜡样芽孢杆菌、链霉菌属、金黄色葡萄球菌和沙门氏菌属）和两种革兰氏阴性菌（大肠杆菌和假单胞菌属）显示出强效抗菌活性。SCP提取物对淋巴瘤U937和白血病细胞（THP1）表现出潜在的抗癌活性。该提取物表现出潜在的抗病毒活性，对单纯疱疹病毒II型（HSV - 2）和腺病毒（Ad7）的选择性指数（SI）分别等于11.30和18.40。结果表明了豌豆副产品在各种应用中的化学和抗氧化活性的更准确信息。发现豌豆副产品的化学成分具有体外抗氧化、抗菌以及抗白血病和淋巴瘤的抗病毒活性。
