Hillman J D, Shivers M
Forsyth Dental Center, Boston, MA 02115.
Arch Oral Biol. 1988;33(6):395-401. doi: 10.1016/0003-9969(88)90196-3.
In vitro, Streptococcus sanguis inhibits the growth of Actinobacillus actinomycetemcomitans, a presumed aetiological agent of localized juvenile periodontitis. When provided with glucose and good aeration, a growing culture of Strep. sanguis was found to produce hydrogen peroxide at concentrations in excess of the maximum LD50 reported for strains of A. actinomycetemcomitans. This concentration of hydrogen peroxide also inhibits the growth of the producer organism. A mutant of Strep. sanguis was isolated that lacked the ability to produce alpha-haemolysis on blood agar. This mutant had less than 3 per cent of its parent's level of pyruvate-oxidase activity, and made no detectable hydrogen peroxide. In vitro, the mutant had also lost the ability to inhibit the growth of A. actinomycetemcomitans. A spontaneous revertant, isolated by its ability to produce alpha-haemolysis, was found to have regained parental levels of pyruvate-oxidase activity and hydrogen-peroxide production and could inhibit the growth of A. actinomycetemcomitans in vitro. A gnotobiotic rat model was used to demonstrate that Strep. sanguis and A. actinomycetemcomitans interact in vivo and that this interaction depends on hydrogen-peroxide production by Strep. sanguis.
在体外，血链球菌可抑制伴放线放线杆菌的生长，后者被认为是局限性青少年牙周炎的一种病因。当提供葡萄糖并保证良好通气时，发现生长中的血链球菌培养物产生的过氧化氢浓度超过了伴放线放线杆菌菌株所报道的最大半数致死剂量。这种过氧化氢浓度也会抑制产生该物质的生物体的生长。分离出一株血链球菌突变体，该突变体在血琼脂上缺乏产生α-溶血的能力。该突变体的丙酮酸氧化酶活性不到其亲本水平的3%，且未检测到过氧化氢的产生。在体外，该突变体也失去了抑制伴放线放线杆菌生长的能力。通过其产生α-溶血的能力分离出的一个自发回复突变体，被发现恢复到了亲本的丙酮酸氧化酶活性和过氧化氢产生水平，并且在体外能够抑制伴放线放线杆菌的生长。使用悉生大鼠模型来证明血链球菌和伴放线放线杆菌在体内相互作用，且这种相互作用取决于血链球菌产生过氧化氢的能力。
