Rahaman Md Motiur, Zwart Rebecca S, Thompson John P
University of Southern Queensland, Centre for Crop Health, Toowoomba, QLD 4350, Australia.
Plants (Basel). 2020 Apr 9;9(4):485. doi: 10.3390/plants9040485.
Plant-derived phenolic compounds contribute to the defense against various pathogens, including root-lesion nematodes ( spp.). However, there are no reports on the role of phenolic compounds in wheat () against . In this study, wheat genotypes ranging from resistant to very susceptible to were used to investigate the level of total phenols and phenol oxidases, polyphenol oxidase (PPO), and peroxidase (POD) expressed in root tissues when grown in the presence and absence of over time (2-8 weeks). Higher constitutive levels of total phenols were found in resistant synthetic hexaploid wheats CPI133872 (576 µg gallic acid equivalent (GAE)/g root) and CPI133859 (518 µg GAE/g root) at 8 weeks after sowing, compared with moderately resistant and susceptible genotypes (192 to 390 µg GAE/g root). The activity of PPO was induced in resistant (CPI133872) and moderately resistant (GS50a and its derivate QT8343) genotypes, becoming maximal at 4 weeks after inoculation. The activity of POD was induced in CPI133872 at 6 weeks after inoculation. Different genetic sources of resistance to showed diverse defense mechanisms and differences in timing responses. The combined effects of total phenols and oxidative enzymes could be important for defense against in some resistant wheat genotypes.
植物源酚类化合物有助于抵御包括根腐线虫（ spp.）在内的各种病原体。然而，关于酚类化合物在小麦（）抵御 方面的作用尚无报道。在本研究中，使用从抗 到极感 的不同小麦基因型，研究在有 和无 的情况下随着时间推移（2 - 8周）根组织中总酚以及酚氧化酶、多酚氧化酶（PPO）和过氧化物酶（POD）的表达水平。与中抗和感病基因型（192至390微克没食子酸当量（GAE）/克根）相比，在播种后8周，抗性合成六倍体小麦CPI133872（576微克GAE/克根）和CPI133859（518微克GAE/克根）中发现了更高的总酚组成水平。PPO的活性在抗性（CPI133872）和中抗（GS50a及其衍生品种QT8343）基因型中被诱导，在接种 后4周达到最大值。POD的活性在接种 后6周在CPI133872中被诱导。不同的抗 基因来源表现出不同的防御机制和时间响应差异。总酚和氧化酶的综合作用可能对一些抗性小麦基因型抵御 很重要。
