Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
Pestic Biochem Physiol. 2020 Mar;164:91-99. doi: 10.1016/j.pestbp.2019.12.013. Epub 2020 Jan 2.
Baculoviruses have been developed as long-term and environmentally friendly biopesticides. However, solar ultraviolet radiation can reduce the activity of baculovirus. Radiation sensitive 23 (Rad23) can recognize DNA damage and is involved with nucleotide excision repair (NER). In the current study, BmRad23 was accumulated mainly within the nucleus. Host cell reactivation (HCR) assays have shown that BmRad23 significantly facilitated the expression of UV-damaged mCherry reporter gene. Reverse transcription quantitative PCR (RT-qPCR) result showed that the mRNA expression level of BmRad23 was increased in (Bombyx mori nuclear polyhedrovirus, BmNPV) BmNPV-infected BmN cells. However, the expression of BmRad23 was increased significantly when BmNPV budded viruses (BVs) or BmN cells were irradiated with UV light. Overexpression of BmRad23 promoted the mRNA levels of two UV-induced DNA damage repair genes which were from Bombyx mori and BmNPV, respectively. Meanwhile, the overexpression of BmRad23 in BmN cells was conducive to the proliferation of BmNPV and UV-damaged BmNPV. The recombinant BmNPV BVs expressing BmRad23 showed stronger resistance to UV radiation than the control virus. In conclusion, the results revealed that BmRad23 contributed to the proliferation of BmNPV and the repair of UV-damaged BmNPV, which would provide a reference for the development of efficient baculovirus pesticides against UV radiation.
杆状病毒已被开发为长期且环保的生物杀虫剂。然而，太阳紫外线辐射会降低杆状病毒的活性。辐射敏感蛋白 23（Rad23）可以识别 DNA 损伤，并参与核苷酸切除修复（NER）。在本研究中，BmRad23 主要积累在细胞核内。宿主细胞复活（HCR）测定表明，BmRad23 显著促进了 UV 损伤的 mCherry 报告基因的表达。反转录定量 PCR（RT-qPCR）结果显示，在 BmNPV 感染的 BmN 细胞中，BmRad23 的 mRNA 表达水平增加。然而，当 BmNPV 出芽病毒（BVs）或 BmN 细胞受到紫外线照射时，BmRad23 的表达显著增加。BmRad23 的过表达促进了两个分别来自家蚕和 BmNPV 的 UV 诱导的 DNA 损伤修复基因的 mRNA 水平。同时，BmN 细胞中 BmRad23 的过表达有利于 BmNPV 和 UV 损伤的 BmNPV 的增殖。表达 BmRad23 的重组 BmNPV BVs 对紫外线辐射的抵抗力强于对照病毒。总之，结果表明 BmRad23 有助于 BmNPV 的增殖和 UV 损伤的 BmNPV 的修复，这将为开发针对紫外线辐射的高效杆状病毒杀虫剂提供参考。
