Department of Biological Sciences, School of Life Sciences, Jiangsu University, Zhenjiang, China.
Insect Mol Biol. 2024 Dec;33(6):638-649. doi: 10.1111/imb.12928. Epub 2024 May 27.
Ribosomal protein L13 (RPL13) is highly conserved in evolution. At present, the properties and functions of RPL13 have not been characterised in insects. In this study, Bombyx mori RPL13 (BmRPL13) was first found to be specifically recruited to the sites of ultraviolet (UV)-induced DNA damage and contributed to UV damage repair. Escherichia coli expressing BmRPL13 showed better resistance to UV radiation. After knocking down the expression of BmRPL13 in BmN cells, the repair speed of UV-damaged DNA slowed down. The further results showed that BmRPL13 interacted with B. mori nucleopolyhedrovirus (BmNPV) ORF65 (Bm65) protein to locate at the UV-induced DNA damage sites of BmNPV and helped repair UV-damaged viral DNA.
核糖体蛋白 L13（RPL13）在进化中高度保守。目前，昆虫中 RPL13 的特性和功能尚未得到表征。在这项研究中，首次发现家蚕 RPL13（BmRPL13）特异性募集到紫外线（UV）诱导的 DNA 损伤部位，并有助于 UV 损伤修复。表达 BmRPL13 的大肠杆菌对紫外线辐射表现出更好的抗性。在 BmN 细胞中敲低 BmRPL13 的表达后，UV 损伤 DNA 的修复速度减慢。进一步的结果表明，BmRPL13 与家蚕核型多角体病毒（BmNPV）ORF65（Bm65）蛋白相互作用，定位在家蚕核型多角体病毒的 UV 诱导的 DNA 损伤部位，并有助于修复 UV 损伤的病毒 DNA。
