College of Health and Rehabilitation Sciences, Department of Health Sciences, Clinical Nutrition, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Environ Sci Pollut Res Int. 2020 Jun;27(17):21892-21904. doi: 10.1007/s11356-020-08445-5. Epub 2020 Apr 13.
Coccinia grandis (C. grandis) L is an Indian medicinal plant from the Cucurbitaceae family whose extracts possess anti-oxidant, anti-infective, and anti-inflammatory properties. The objective of the present study was to probe the potential immunomodulatory of C. grandis crude extract on different pathways in THP-1 cells as probed by changes in expression of several proteins. THP-1 cells were differentiated into macrophages after treatment with phorbol-12-myristate 13-acetate, followed by exposure to lipopolysaccharide (LPS) with or without 50 or 100 μg/ml of C. grandis extract. Treatment of the cells with the extract significantly downregulated the expression and release of pro-inflammatory cytokines (IL-6, IL-1β, CCL2, CCL22, CXCL10/IP-10, CX3CL1 and CXCL8/IL-8), proteins (ERK5, BAX, BCL2, Cyclin D, ERK1, NF-κB, P-IκBα,P- NF-κB and P-p38) and molecular signaling pathways (NF-κB, p38 MAPK, ERK1/2 and IL-6/JAK/STAT3 signaling cascades). This study is the first to highlight the ability of C. grandis extract to modulate several pathways, including proliferation, the expression of inflammatory cytokines, phagocytosis, migration properties and apoptosis, in human monocytic THP-1 cells.
冬瓜子（C. grandis）L 是葫芦科的一种印度药用植物，其提取物具有抗氧化、抗感染和抗炎特性。本研究的目的是探讨 C. grandis 粗提取物对 THP-1 细胞中不同途径的潜在免疫调节作用，方法是检测几种蛋白质表达的变化。用佛波醇 12-肉豆蔻酸 13-乙酸处理 THP-1 细胞使其分化为巨噬细胞，然后用脂多糖（LPS）处理，或用 50 或 100μg/ml 的 C. grandis 提取物处理。细胞提取物处理显著下调促炎细胞因子（IL-6、IL-1β、CCL2、CCL22、CXCL10/IP-10、CX3CL1 和 CXCL8/IL-8）、蛋白（ERK5、BAX、BCL2、Cyclin D、ERK1、NF-κB、P-IκBα、P-NF-κB 和 P-p38）和分子信号通路（NF-κB、p38 MAPK、ERK1/2 和 IL-6/JAK/STAT3 信号级联）的表达和释放。本研究首次强调了 C. grandis 提取物能够调节几种途径的能力，包括人单核细胞 THP-1 细胞的增殖、炎症细胞因子表达、吞噬作用、迁移特性和细胞凋亡。
