College of Health and Rehabilitation Sciences, Department of Health Sciences, Clinical Nutrition, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Oxid Med Cell Longev. 2020 Sep 25;2020:7574606. doi: 10.1155/2020/7574606. eCollection 2020.
() is a plant from the Compositae family that exhibits antioxidant, anti-inflammatory, antibacterial, and cytotoxic activities.
The current study aimed at investigating the immunomodulatory effects of extract in lipopolysaccharide- (LPS-) stimulated human monocytic THP-1 cells.
To induce macrophage differentiation, THP-1 cell lines were treated with phorbol-12-myristate 13-acetate, followed by exposure to LPS with or without 50 or 100 g/ml of extract. The following tests were employed to test the immunomodulatory effects of the extract: MTT assay, ELISA, Western blotting analysis, cell migration and phagocytosis assays, and Annexin V staining method.
Exposure to 100 g/ml extract significantly reduced THP-1 cell proliferation, migration, and phagocytosis (in LPS-stimulated cells, but not in unstimulated cells). Moreover, the extract alone significantly reduced the rate of THP-1 cell apoptosis, while it increased the rate of late apoptosis. Molecular investigations showed that treatment with extract significantly upregulated the expression of ERK1, p-MAPK, P-P38, and Bcl2, while it significantly reduced the expression of ERK5, Bax, NF-B, P-NF-B, CCL1, CCL2, CCL5, CCL22, CXCL1, and CXCL10.
extract exhibited anti-inflammatory, antiproliferative, antimigratory, and antiphagocytic effects in LPS-stimulated THP-1 cells. Future studies should investigate these mechanisms in animal models with chronic inflammatory diseases.
（）是菊科植物，具有抗氧化、抗炎、抗菌和细胞毒性作用。
本研究旨在探讨 提取物对脂多糖（LPS）刺激的人单核细胞 THP-1 细胞的免疫调节作用。
为诱导巨噬细胞分化，用佛波醇 12-肉豆蔻酸 13-乙酸酯处理 THP-1 细胞系，然后用 LPS （或不用）和 50 或 100μg/ml 的 提取物处理。采用 MTT 检测、ELISA、Western blot 分析、细胞迁移和吞噬试验以及 Annexin V 染色法检测提取物的免疫调节作用。
暴露于 100μg/ml 提取物显著降低 LPS 刺激的 THP-1 细胞增殖、迁移和吞噬作用（但不影响未刺激的细胞）。此外，提取物本身可显著降低 THP-1 细胞凋亡率，同时增加晚期凋亡率。分子研究表明，用 提取物处理可显著上调 ERK1、p-MAPK、P-P38 和 Bcl2 的表达，同时显著降低 ERK5、Bax、NF-B、P-NF-B、CCL1、CCL2、CCL5、CCL22、CXCL1 和 CXCL10 的表达。
提取物对 LPS 刺激的 THP-1 细胞表现出抗炎、抗增殖、抗迁移和抗吞噬作用。未来的研究应在慢性炎症性疾病的动物模型中研究这些机制。
