LncRNA promotes tumour progression through /Wnt/β-catenin signalling pathway in hepatocellular carcinoma.

The present study aimed to investigate the function of lncRNA (cancer susceptibility candidate 15) in hepatocellular carcinoma (HCC), as well as its regulatory roles in expression and Wnt/β-catenin pathway. Quantitative real-time polymerase chain reaction (QRT-PCR) method was used to detect the relative expression of mRNA in HCC tissues. Protein detection was performed by western blot. Luciferase assay was used to confirm the potential target of in HCC. Cell proliferation, migration and invasion, as well as apoptosis were analyzed using MTT, transwell assays and flow cytometry in vitro, respectively. The expression of was significantly increased in HCC tissues ( < .001) and showed positive correlation with tumour size ( = .016), TNM stage ( = .018) and metastasis ( = .021). The knockdown of could obviously inhibit HCC cell proliferation, migration and invasion and promote cell apoptosis in vitro ( < .05 for all). Furthermore, the protein levels of , β-catenin, Cyclin D1 and c-Myc also exhibited decreased trends after inhibition. Luciferase assay confirmed that might be a targeted gene of in HCC. In HCC, may activate the Wnt/β-catenin pathway via enhancing the expression of , thus promote tumour progression.