Sun Qingshen, Han Dequan, Lei Hong, Zhao Kai, Zhu Li, Li Xiaodi, Fu Honggang
1Key Laboratory of Functional Inorganic Material Chemistry, Heilongjiang University, Ministry of Education, Harbin, 150080 China.
2University Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, 150080 China.
J Wuhan Univ Technol Mater Sci Ed. 2012;27(3):459-464. doi: 10.1007/s11595-012-0485-4. Epub 2012 Jun 9.
To optimize the preparation process of chitosan microspheres and study its loading capacity, chitosan microsphere was prepared by crosslinking with glutaraldehyde, and bovine serum albumin (BSA) was absorbed onto chitosan microsphere. Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FITR), TA instruments and zeta potentiometer analyzer were used to characterize the parameters with respect to size, thermal characters, morphology, and zeta potential of the microspheres. The loading capability and release tests were carried out. The results showed that chitosan microsphere with particle size less than 10 μm and positively charged (+25.97±0.56 mV) can be obtained under the aldehyde group to amino group ratio at 1:1. A loading capacity of BSA at 28.63±0.15 g/100 g with corresponding loading efficiency at 72.01±1.44% was obtained for chitosan microsphere. test revealed a burst release followed by sustained-release profile.
为优化壳聚糖微球的制备工艺并研究其载药量，采用戊二醛交联法制备壳聚糖微球，并将牛血清白蛋白（BSA）吸附到壳聚糖微球上。利用扫描电子显微镜（SEM）、傅里叶变换红外光谱仪（FITR）、TA仪器和zeta电位分析仪对微球的尺寸、热性质、形态和zeta电位等参数进行表征。进行了载药能力和释放试验。结果表明，在醛基与氨基比例为1:1的条件下，可获得粒径小于10μm且带正电（+25.97±0.56 mV）的壳聚糖微球。壳聚糖微球对BSA的载药量为28.63±0.15 g/100 g，相应的载药效率为72.01±1.44%。试验显示出先有突释然后是缓释的曲线。
