Wang Shuai, Zhang Meng, Liu Xin-Chao, Lin Tao, Yang Han-Chun, Yuan Shi-Shan, Zhao Guang-Wei, Ia Hassan, Yan Ruo-Feng, Song Xiao-Kai, Xu Li-Xin, Li Xiang-Rui
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China.
Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R.China.
J Integr Agric. 2015 Sep;14(9):1838-1844. doi: 10.1016/S2095-3119(15)61044-9. Epub 2015 Sep 6.
The objective of the present investigation was to estimate the prevalence of infection and co-infection with porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV) and porcine circovirus type 2 (PCV-2) in pigs in China. A total of 372 tissues or serum samples collected from pigs distributed in 9 provinces/municipalities of China during the period from February 2011 to November 2012 were assayed for antigens and antibodies using enzyme linked immunosorbent assay (ELISA) technique, while the PCR was designed for the detection of the PRRSV, CSFV and PCV-2, respectively. The total positive rate of PRSSV, CSFV and PCV-2 was 9.14% (34/372), 50.00% (186/372), 37.10% (138/372) and 3.23% (12/372), respectively. Among the 34 positive samples, 26 samples were simultaneously infected with and viruses, while the remaining eight samples were infected with alone. In addition, the co-infection rate of with PRSSV, with PRSSV and CSFV, with PRSSV and PCV-2, with CSFV and PCV-2, with PRSSV, CSFV and PCV-2 was 1.61% (6/372), 4.03% (15/372), 0.27% (1/372), 0.27% (1/372) and 0.81% (3/372), respectively. The results of the present survey revealed that PRRSV and CSFV were the common pathogens co-existing with porcine toxoplasmosis in China, and both of them could increase the chances of infection in pig. This is the first report of co-infections with viruses in pigs. It is very important to understand the interactions of parasite and virus, and can be used as reference data for the control and prevention of co-infections of and viruses in pigs.
本研究的目的是估计中国猪群中猪繁殖与呼吸综合征病毒（PRRSV）、经典猪瘟病毒（CSFV）和猪圆环病毒2型（PCV - 2）的感染率和混合感染率。在2011年2月至2012年11月期间，从中国9个省/直辖市的猪群中采集了372份组织或血清样本，采用酶联免疫吸附测定（ELISA）技术检测抗原和抗体，同时设计了PCR分别用于检测PRRSV、CSFV和PCV - 2。PRRSV、CSFV和PCV - 2的总阳性率分别为9.14%（34/372）、50.00%（186/372）、37.10%（138/372）和3.23%（12/372）。在34份阳性样本中，26份样本同时感染了两种病毒，其余8份样本仅感染了一种病毒。此外，PRRSV与CSFV、PRRSV与PCV - 2、CSFV与PCV - 2、PRRSV、CSFV与PCV - 2的混合感染率分别为1.61%（6/372）、4.03%（15/372）、0.27%（1/372）、0.27%（1/372）和0.81%（3/372）。本次调查结果显示，PRRSV和CSFV是中国猪群中与猪弓形虫病共存的常见病原体，它们均可增加猪感染的几率。这是关于猪群中病毒混合感染的首次报道。了解寄生虫与病毒的相互作用非常重要，可为猪群中寄生虫与病毒混合感染的防控提供参考数据。
