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基于循环抗原和抗体检测中国散养鸡肉中的弓形虫。

Detection of Toxoplasma gondii in free-range chickens in China based on circulating antigens and antibodies.

机构信息

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.

出版信息

Vet Parasitol. 2012 Apr 30;185(2-4):72-7. doi: 10.1016/j.vetpar.2011.10.031. Epub 2011 Nov 11.

DOI:10.1016/j.vetpar.2011.10.031
PMID:22153258
Abstract

Toxoplasma gondii is widely distributed in humans and other animals including domestic poultry throughout the world, but the data on prevalence of T. gondii in free-ranged (FR) chickens in People's Republic of China (PRC) are limited. In the present study, the seroprevalence of T. gondii infection in FR chickens was investigated in 13 provinces/municipalities of China during the period from January to June 2010. A total of 1173 serum samples were collected and assayed for T. gondii circulating antigens (TCA) and antibodies (TCAb) using enzyme linked immunosorbent assay (ELISA) technique. Out of this number, 199 samples were TCA positive (16.97%), 226 samples were TCAb positive (19.27%), 69 samples were positive for both TCA and TCAb (5.88%), and the total seropositive rate was found in 356 of 1173 (30.36%). The results of the present survey indicated that infection with T. gondii in FR chickens is widely spread in China.

摘要

刚地弓形虫广泛分布于全世界的人类和其他动物中,包括家禽,但目前有关中国(中华人民共和国)自由放养(FR)鸡弓形虫感染流行率的数据有限。本研究于 2010 年 1 月至 6 月期间在中国 13 个省/直辖市对 FR 鸡弓形虫感染的血清流行率进行了调查。共采集了 1173 份血清样本,使用酶联免疫吸附试验(ELISA)技术检测弓形虫循环抗原(TCA)和抗体(TCAb)。在这些样本中,199 份 TCA 阳性(16.97%),226 份 TCAb 阳性(19.27%),69 份 TCA 和 TCAb 均阳性(5.88%),1173 份样本中有 356 份总血清阳性(30.36%)。本调查结果表明,中国 FR 鸡弓形虫感染广泛流行。

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