Yooklaen Juthatip, Srisawat Theera, Khang Luu Tang Phuc, Dinh-Hung Nguyen, Sangsawad Papungkorn, Linh Nguyen Vu, Permpoonpattana Patima
Department of Agricultural Science and Technology, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani, Thailand.
Department of Agricultural Science and Technology, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani, Thailand.
Int J Microbiol. 2025 Jun 30;2025:7471536. doi: 10.1155/ijm/7471536. eCollection 2025.
, an endemic species of the Dipterocarpaceae family, possesses notable medicinal properties. However, its application as an antibacterial agent is limited due to the insufficient investigations of its antibacterial activity from flower extracts. This study is aimed at exploring the antibacterial mechanisms of acetone extracts from the flowers of against four bacterial strains using various methods, including the well-disk diffusion assay, minimum inhibitory concentration (MIC) determination, minimum bactericidal concentration (MBC) assessment, flow cytometry, and scanning electron microscopy. The inhibition zones measured between 6.33 and 17.66 mm. Notably, the extract exhibited different MIC values, such as 250 g mL for and , and only 62.5 g.mL for , demonstrating its effectiveness. MBC values ranged from 500 to over 1000 g mL for . Flow cytometric analysis revealed that the cellular responses to the extract were influenced by both the concentration of the extract and the duration of exposure, indicating a dose- and time-dependent antibacterial effect. Additionally, scanning electron microscopy confirmed that the extract caused structural damage to the cells of both Gram-positive and Gram-negative bacteria. Overall, this study underscores the promising antibacterial potential of flower extracts, which demonstrate significant efficacy against a variety of bacterial strains.
（某植物）是龙脑香科的一种特有物种，具有显著的药用特性。然而，由于对其花提取物的抗菌活性研究不足，其作为抗菌剂的应用受到限制。本研究旨在通过多种方法，包括纸片扩散法、最低抑菌浓度（MIC）测定、最低杀菌浓度（MBC）评估、流式细胞术和扫描电子显微镜，探索（某植物）花的丙酮提取物对四种细菌菌株的抗菌机制。抑菌圈直径在6.33至17.66毫米之间。值得注意的是，提取物表现出不同的MIC值，例如对（某两种细菌）为250微克/毫升，而对（另一种细菌）仅为62.5微克/毫升，证明了其有效性。（某细菌）的MBC值范围为500至超过1000微克/毫升。流式细胞术分析表明，细胞对提取物的反应受提取物浓度和暴露时间的影响，表明具有剂量和时间依赖性的抗菌作用。此外，扫描电子显微镜证实，提取物对革兰氏阳性菌和革兰氏阴性菌的细胞均造成了结构损伤。总体而言，本研究强调了（某植物）花提取物具有有前景的抗菌潜力，其对多种细菌菌株显示出显著疗效。
