REQUIMTE/LAQV, Instituto Superior de Engenharia do Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, Portugal.
REQUIMTE/LAQV, Departamento. de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
Molecules. 2020 Apr 10;25(7):1739. doi: 10.3390/molecules25071739.
Wine production generates large amounts of vine-canes, a devalued by-product that could be used for the recovery of bioactive compounds. In this work, two vine-canes varieties, namely Touriga Nacional (TN) and Tinta Roriz (TR), were submitted to different ultrasound-assisted extraction (UAE) conditions. The highest phenolic and flavonoid content was observed for TR extract obtained at lab-scale without an ice bath and pilot-scale after 60 min of extraction (32.6 ± 2.1 and 26.0 ± 1.5 mg gallic acid equivalent/g dry weight (dw) and 9.5 ± 0.6 and 8.3 ± 0.8 mg epicatechin equivalents/g dw, respectively). Further, all extracts demonstrated a high antioxidant activity to scavenge DPPH free radicals with the best value reached by TR at the lab-scale without an ice bath after 30 min and pilot-scale extraction after 60 min (34.2 ± 2.4 and 33.4 ± 2.1 mg trolox equivalents/g dw, respectively). Extracts phenolic composition were also evaluated by HPLC, demonstrating that resveratrol, myricetin and catechin were the main compounds. According to our knowledge, this is the first time that a pilot scale of UAE of phenolic compounds from vine-canes was performed. This paper represents an important step to the use of UAE as an industrial process to recover bioactive compounds.
葡萄酒生产会产生大量的葡萄藤废料，这是一种被低估的副产物，但可以用于回收生物活性化合物。在这项工作中，我们对两种葡萄藤品种，即国产多瑞加（Touriga Nacional，TN）和国产红（Tinta Roriz，TR），进行了不同的超声辅助提取（UAE）条件处理。在实验室规模下没有冰浴和中试规模下提取 60 分钟后，TR 提取物的酚类和类黄酮含量最高（分别为 32.6±2.1 和 26.0±1.5mg 没食子酸当量/g 干重（dw）和 9.5±0.6 和 8.3±0.8mg 表儿茶素当量/g dw）。此外，所有提取物均表现出较高的抗氧化活性，可清除 DPPH 自由基，其中以实验室规模下没有冰浴提取 30 分钟和中试规模下提取 60 分钟后的 TR 提取物效果最佳（分别为 34.2±2.4 和 33.4±2.1mg trolox 当量/g dw）。通过 HPLC 对提取物的酚类成分进行评价，结果表明白藜芦醇、杨梅素和儿茶素是主要化合物。据我们所知，这是首次对葡萄藤废料中的酚类化合物进行中试规模的 UAE。本文代表了将 UAE 用作回收生物活性化合物的工业过程的重要一步。
