灌注人胎盘小叶的物理完整性和代谢活力标志物。

Markers of physical integrity and metabolic viability of the perfused human placental lobule.

Cannell G R, Kluck R M, Hamilton S E, Mortimer R H, Hooper W D, Dickinson R G

Conjoint Internal Medicine Laboratory, Royal Brisbane Hospital, Queensland, Australia.

Clin Exp Pharmacol Physiol. 1988 Nov;15(11):837-44. doi: 10.1111/j.1440-1681.1988.tb01026.x.

Abstract

Peripheral lobules of term placentae obtained from healthy females at Caesarian section were perfused using separate maternal and fetal circulations for 6 h periods under either oxygenated or anoxic conditions. 2. Markers of physical integrity during setting-up and initial perfusion were establishment of dual perfusion within 25 min of placental delivery, pressure in the fetal capillary network less than 40 mmHg, leakage of perfusate from fetal to maternal compartments less than or equal to 2 ml/h, and overlap of maternal with fetal perfusion as indicated visually by appropriate blanching and verified by a fetal artery to vein oxygen gradient of greater than or equal to 90 mmHg. 3. Post-perfusion markers of metabolic viability were most reliably indicated by glucose consumption (oxygenated 7.8 +/- 1.5, anoxic 17.7 +/- 1.2 mmol/kg per h), lactate production (oxygenated 8.5 +/- 1.4, anoxic 33.9 +/- 2.5 mmol/kg per h) and human placental lactogen production (oxygenated 41.2 +/- 9.8, anoxic 12.2 +/- 3.4 mg/kg per h).

摘要

在剖宫产时，从健康女性获取足月胎盘的外周小叶，在有氧或缺氧条件下，分别通过母体和胎儿循环进行6小时灌注。2. 建立和初始灌注期间物理完整性的指标包括：胎盘娩出后25分钟内建立双循环灌注、胎儿毛细血管网络压力小于40 mmHg、灌注液从胎儿腔室漏至母体腔室的量小于或等于2 ml/h，以及通过适当的皮肤变白在视觉上显示并经胎儿动静脉氧梯度大于或等于90 mmHg证实的母体与胎儿灌注重叠。3. 灌注后代谢活力的指标最可靠地由葡萄糖消耗（有氧7.8±1.5，缺氧17.7±1.2 mmol/kg每小时）、乳酸产生（有氧8.5±1.4，缺氧33.9±2.5 mmol/kg每小时）和人胎盘催乳素产生（有氧41.2±9.8，缺氧12.2±3.4 mg/kg每小时）来表明。