Villano Clizia, D'Amelia Vincenzo, Esposito Salvatore, Adelfi Maria Grazia, Contaldi Felice, Ferracane Rosalia, Vitaglione Paola, Aversano Riccardo, Carputo Domenico
Department of Agricultural Sciences, University of Naples Federico II, Via Università 133, 80055 Portici NA, Italy.
National Research Council of Italy, Institute of Biosciences and Bioresources (CNR-IBBR), Via Università 133, 80055 Portici NA, Italy.
Life (Basel). 2020 Apr 10;10(4):37. doi: 10.3390/life10040037.
Steroidal glycoalkaloids (SGAs) are a class of nitrogen-containing glycosides occurring in several plant families and biosynthesized through a specific pathway. HMG-CoA reductase is the first enzyme of this pathway, and its transcription can be regulated by biotic and abiotic stressors and even in a tissue-specific manner. This study aimed to characterize the genes family in a tuber-bearing potato species, , using transcriptional and functional approaches. Our results provided evidence that four with different tissue-specificities represent the gene family in and that they originated from through segmental duplications. Phylogenetic analysis suggests that is the direct ortholog of , which is associated with SGAs accumulation in plants. Its overexpression in revealed that this gene plays a key role in the accumulation of glycoalkaloids regulating the production of dehydrocommersonine.
甾体糖苷生物碱（SGAs）是一类含氮糖苷，存在于多个植物科中，并通过特定途径生物合成。HMG-CoA还原酶是该途径的第一个酶，其转录可受到生物和非生物胁迫因子的调控，甚至具有组织特异性。本研究旨在利用转录和功能方法，对一种块茎马铃薯物种中的基因家族进行表征。我们的结果表明，四个具有不同组织特异性的基因代表了该物种中的基因家族，并且它们通过片段重复起源于。系统发育分析表明，是与植物中SGAs积累相关的的直系同源基因。它在中的过表达表明，该基因在调节脱氢番茄碱生产的糖苷生物碱积累中起关键作用。
