Department of Biotechnology and Food Science, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway.
NOFIMA, 6600 Sunndalsøra, Norway.
Molecules. 2020 Apr 12;25(8):1769. doi: 10.3390/molecules25081769.
This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra.
本研究旨在评估不同饲料（富含海洋、贫瘠、贫瘠添加海洋磷脂（MPL）和贫瘠添加二十二碳六烯酸（DHA））喂养的养殖大西洋三文鱼分离的肝细胞中虾青素（Ax）的积累情况。采用核磁共振（NMR）光谱法检测和定量分析 Ax。使用 C 富集的 Ax 可以评估 Ax 的短期代谢情况。在鱼类饲料中用植物类似物替代鱼油和鱼粉，并添加 MPL 会导致肝细胞中 Ax 的进一步分解代谢和积累减少，从 17 毫克/千克降至约 6 毫克/千克，添加 DHA 则几乎降至零。使用一维和二维 NMR 光谱对丙酮中天然和 C 富集虾青素的信号进行了分配。
