Mun Bong-Gyu, Lee Sang-Uk, Hussain Adil, Kim Hyun-Ho, Rolly Nkulu Kabange, Jung Ki-Hong, Yun Byung-Wook
School of Applied Bioscience, College of Agriculture and Life Science, Kyungpook National University, 80 Daehak-ro, Bukgu, Daegu, 41566, South Korea.
Department of Plant Molecular Systems Biotechnology and Crop Biotechnology Institute, Kyung Hee University, Giheung-gu, Yongin-si, Gyeonggi-do, 17104, Republic of Korea.
Funct Plant Biol. 2018 May;45(6):630-644. doi: 10.1071/FP17249.
Rice (Oryza sativa L.) is a major food crop and also a well-established genetic model. Nitric oxide (NO) and its derivatives are important signalling molecules that actively participate in various signalling pathways in response to different stresses. In this study, we performed RNA-seq mediated transcriptomic analysis of rice after treatment with the nitric oxide donor, S-nitroso-L-cysteine (CySNO), generating an average of 37.5 and 41.5 million reads from control and treated leaf samples respectively. More than 95% of the reads were successfully mapped to the O. sativa reference genome yielding a total of 33539 differentially expressed genes (DEGs, P < 0.05). Further analyses identified 825 genes with at least 2-fold change in the expression following treatment with CySNO (P < 0.01). The DEGs identified were involved in diverse molecular functions such as catalytic activity, binding, transport, and receptor activity and were mostly located in the membrane, organelles such as nucleus, Golgi apparatus and mitochondria. DEGs also contained several genes that regulate responses to abiotic stresses such as drought, heat, cold and salt stress and biotic stresses. We also found significantly similar expression patterns of CySNO-responsive DEGs of rice with the CySNO-responsive DEGs of Arabidopsis in a previous study. Expression patterns of genes involved in key biological functions were verified using quantitative real time (qRT)-PCR. The findings of this study suggest that NO regulates the transcriptional control of genes involved in a wide variety of physiological functions in rice, and that NO-mediated transcriptional networks are highly conserved across the plant kingdom. This study provides useful information regarding the transcriptional response of plants to nitrosative stress.
水稻（Oryza sativa L.）是一种主要的粮食作物，也是一个成熟的遗传模型。一氧化氮（NO）及其衍生物是重要的信号分子，积极参与各种响应不同胁迫的信号通路。在本研究中，我们对用一氧化氮供体S-亚硝基-L-半胱氨酸（CySNO）处理后的水稻进行了RNA测序介导的转录组分析，分别从对照和处理后的叶片样本中平均获得了3750万和4150万条 reads。超过95%的 reads 成功映射到水稻参考基因组，共产生33539个差异表达基因（DEG，P < 0.05）。进一步分析确定了825个基因，在用CySNO处理后表达变化至少2倍（P < 0.01）。所鉴定的DEG参与了多种分子功能，如催化活性、结合、转运和受体活性，且大多位于膜、细胞核、高尔基体和线粒体等细胞器中。DEG还包含几个调节对干旱、高温、低温和盐胁迫等非生物胁迫以及生物胁迫响应的基因。我们还发现，在之前的一项研究中，水稻对CySNO响应的DEG与拟南芥对CySNO响应的DEG具有显著相似的表达模式。使用定量实时（qRT）-PCR验证了参与关键生物学功能的基因的表达模式。本研究结果表明，NO调节水稻中参与多种生理功能的基因的转录控制，并且NO介导的转录网络在植物界高度保守。本研究提供了有关植物对亚硝化胁迫转录反应的有用信息。
