Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea.
Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea.
Int J Mol Sci. 2021 Oct 26;22(21):11541. doi: 10.3390/ijms222111541.
The intrinsic defense mechanisms of plants toward pathogenic bacteria have been widely investigated for years and are still at the center of interest in plant biosciences research. This study investigated the role of the gene encoding a transcription factor (TF) in the basal defense and systemic acquired resistance in Arabidopsis using the reverse genetics approach. To achieve that, the mutant line (lacking the gene) was challenged with pv. () inoculated by infiltration into Arabidopsis leaves at the rosette stage. The results indicated that plants showed an enhanced resistance phenotype toward over time compared to Col-0 and the susceptible disease controls, and In addition, the transcript accumulation of pathogenesis-related genes, and , increased significantly in over time (0-72 h post-inoculation, hpi) compared to that of and (susceptible lines), with prevailing over . When coupled with the recorded pathogen growth (expressed as a colony-forming unit, CFU mL), the induction of genes, associated with the salicylic acid (SA) defense signaling, in part explained the observed enhanced resistance of mutant plants in response to . Furthermore, when was inoculated, the expression of was upregulated in the systemic leaves of Col-0, while that of remained at a basal level in Col-0. Moreover, the expression of (a systemic acquired resistance -related) gene was significantly upregulated at all time points (0-24 h post-inoculation, hpi) in compared to Col-0 and and . Under the same conditions, exhibited a high transcript accumulation level 48 hpi in the background. Therefore, all data put together suggest that and coupled with and , with prevailing over , would contribute to the recorded enhanced resistance phenotype of the mutant line against . Thus, the TF is proposed as a negative regulator of basal defense and systemic acquired resistance in plants under infection.
多年来，人们广泛研究了植物对病原菌的固有防御机制，这些机制仍然是植物生物科学研究的核心关注点。本研究采用反向遗传学方法，研究了编码转录因子 (TF) 的 基因在拟南芥基础防御和系统获得性抗性中的作用。为了实现这一目标，使用渗透法将 pv. （）接种到拟南芥莲座叶期叶片中，对 突变体系（缺乏 基因）进行了挑战。结果表明，与 Col-0 和易感病对照 相比， 植株随着时间的推移对 表现出增强的抗性表型， 此外，与 Col-0 和 （易感病系）相比，病程相关基因 、 和 的转录积累随着时间的推移（接种后 0-72 小时，hpi）显著增加， 占主导地位。当与记录的病原体生长（表示为菌落形成单位，CFU mL）相结合时，与水杨酸 (SA) 防御信号相关的 基因的诱导部分解释了 突变体植物对 的增强抗性。此外，当接种 时，Col-0 中 的表达在系统叶片中上调，而 Col-0 中 的表达保持在基础水平。此外，与 Col-0 和 相比，在所有时间点（接种后 0-24 小时，hpi）， （与系统获得性抗性相关）基因的表达在 中显著上调，在相同条件下， 背景下的 48 hpi 转录物积累水平较高。因此，所有数据表明， 与 以及 与 相结合，且 占主导地位，将有助于记录到的 突变体系对 的增强抗性表型。因此， 转录因子被提出为植物在 感染下基础防御和系统获得性抗性的负调节剂。
