Xiao Xi, Sticht Carsten, Yin Libo, Liu Li, Karakhanova Svetlana, Yin Yefeng, Georgikou Christina, Gladkich Jury, Gross Wolfgang, Gretz Norbert, Herr Ingrid
Molecular OncoSurgery Group, Section of Surgical Research, Department of General, Visceral and Transplant Surgery, University of Heidelberg, Heidelberg, Germany.
These authors contributed equally to this work and share the first authorship.
Oncotarget. 2020 Apr 7;11(14):1203-1217. doi: 10.18632/oncotarget.27527.
Food-derived plant microRNAs are suggested to control human genes by "cross-kingdom" regulation. We examined microRNAs in sprouts from , known as broccoletti, which are widely used as sulforaphane supplements, and assessed their influence on pancreatic cancer. RNA was isolated from 4-day-old sprouts, followed by deep sequencing and bioinformatic analysis. We identified 2 new and 745 known plant microRNA sequences in the miRbase database and predicted 15,494 human target genes and 76,747 putative 3'-UTR binding sites in these target genes. The most promising candidates were the already known microRNA sequence bra-miR156g-5p and the new sequence Myseq-330, both with predicted human target genes related to apoptosis. The overexpression of the respective oligonucleotides by lipofection did not alter the viability, apoptosis, clonogenicity, migration or associated protein expression patterns in pancreatic cancer cells. These data demonstrate that broccoletti sprouts contain microRNA sequences with putative binding sites in human genes, but the sequences evaluated here did not affect cancer growth. Our database of broccoletti-derived microRNA sequences provides a valuable tool for future analysis.
食物来源的植物微小RNA被认为可通过“跨界”调控来控制人类基因。我们检测了一种名为broccoletti（也被广泛用作萝卜硫素补充剂）的芽苗菜中的微小RNA，并评估了它们对胰腺癌的影响。从4日龄的芽苗菜中分离RNA，随后进行深度测序和生物信息学分析。我们在miRbase数据库中鉴定出2个新的和745个已知的植物微小RNA序列，并预测了15494个人类靶基因以及这些靶基因中的76747个推定的3'-UTR结合位点。最有前景的候选序列是已知的微小RNA序列bra-miR156g-5p和新序列Myseq-330，两者都有与凋亡相关的预测人类靶基因。通过脂质转染过表达各自的寡核苷酸并没有改变胰腺癌细胞的活力、凋亡、克隆形成能力、迁移或相关蛋白表达模式。这些数据表明，broccoletti芽苗菜含有在人类基因中具有推定结合位点的微小RNA序列，但此处评估的序列并未影响癌症生长。我们的broccoletti来源的微小RNA序列数据库为未来的分析提供了一个有价值的工具。
