Yuan Kaisong, de la Asunción-Nadal Victor, Li Yuliang, Jurado-Sánchez Beatriz, Escarpa Alberto
Department of Analytical Chemistry, Physical Chemistry, and Chemical Engineering, University of Alcala, Alcala de Henares, 28871 Madrid (Spain), University of Alcala, 28807, Madrid, Spain.
Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, P. R. China.
Chemistry. 2020 Jul 8;26(38):8471-8477. doi: 10.1002/chem.202001754. Epub 2020 Jun 16.
Graphdiyne (GDY), a new kind of two-dimensional (2D) material, was combined with micromotor technology for "on-the-fly" operations in complex biomedia. Microtubular structures were prepared by template deposition on membrane templates, resulting in functional structures rich in sp and sp carbons with highly conjugated π networks. This resulted in a highly increased surface area for a higher loading of anticancer drugs or enhanced quenching ability over other 2D based micromotors, such as graphene oxide (GO) or smooth tubular micromotors. High biocompatibility with almost 100 % cell viability was observed in cytotoxicity assays with moving micromotors in the presence of HeLa cells. On a first example, GDY micromotors loaded with doxorubicin (DOX) were used for pH responsive release and HeLa cancer cells killing. The use of affinity peptide engineered GDY micromotors was also illustrated for highly sensitive and selective fluorescent OFF-ON detection of cholera toxin B through specific recognition of the subunit B region of the target toxin. The new developments illustrated here offer considerable promise for the use of GDY as part of micromotors in living biosystems.
石墨炔（GDY）是一种新型二维材料，它与微马达技术相结合，可在复杂生物介质中进行“即时”操作。通过在膜模板上进行模板沉积制备微管结构，从而得到富含sp和sp²碳且具有高度共轭π网络的功能结构。这使得其表面积大幅增加，能够更高负载抗癌药物，或比其他基于二维材料的微马达（如氧化石墨烯（GO）或光滑管状微马达）具有更强的猝灭能力。在含有HeLa细胞的细胞毒性试验中，移动微马达表现出几乎100%的细胞活力，具有高生物相容性。在第一个示例中，负载阿霉素（DOX）的GDY微马达用于pH响应释放和杀死HeLa癌细胞。还展示了使用亲和肽工程化的GDY微马达，通过特异性识别目标毒素的B亚基区域，对霍乱毒素B进行高灵敏度和选择性的荧光开-关检测。此处展示的新进展为将GDY用作活体生物系统中微马达的一部分带来了巨大前景。
