Passarelli Pier Carmine, De Leonardis Marta, Piccirillo Giovan Battista, Desantis Viviana, Papa Raffaele, Rella Edoardo, Bonaviri Giuseppe Niccolò Mastandrea, Papi Piero, Pompa Giorgio, Pasquantonio Guido, Manicone Paolo Francesco, D'Addona Antonio
Department of Head and Neck, Division of Oral Surgery and Implantology, Institute of Clinical Dentistry, Università Cattolica del Sacro Cuore, Fondazione Policlinico Universitario Gemelli, 00168 Rome, Italy.
Medicine and Surgery, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
Antibiotics (Basel). 2020 Apr 14;9(4):179. doi: 10.3390/antibiotics9040179.
Peri-implantitis is an inflammatory disease with an increasing diffusion rate which can affect the long-term survival of a prosthetic rehabilitation. The present study focused on the decontaminating efficacy of chlorhexidine and air polishing system with sodium bicarbonate powder against , a microorganism which seems to have a superinfecting opportunistic role in the pathology. The aim of the authors was to investigate and compare the effectiveness of these treatments, commonly used in clinical practice. Methods: An in vitro study was conducted to analyze the effects of two widely used therapeutic aids for the disinfection of affected titanium implants: chlorhexidine (CHX) and air polishing with sodium bicarbonate powder (P). A qualitative and quantitative comparative analysis of the residual biofilm was carried out using a colorimetric assay (XTT) and scanning electron microscopy (SEM) observation. The experiment was conducted both on machined titanium surfaces and on rough sandblasted ones with the aim of bringing out differences in the therapeutic outcomes concerning the superficial texture of the implant. The null hypothesis was that no difference could be detected between the samples, regarding both the treatments performed and the nano-structural features of titanium. The best results (on both types of implant surfaces) were obtained when combining the use of chlorhexidine and air polishing (C + P). A linear decrease in the optical density (OD) values recorded at three different time points (30 s, 1 min, 5 min) was also observed passing from the first to the last one. When observed under scanning electron microscope rough surfaces showed an extensive and highly structured biofilm, more complex if compared to the one encountered when analyzing machined implants. Conclusions: the present pilot study showed that rough surfaces can promote fungal adhesion and eventually hinder the outcome of a decontaminating treatment. For this purpose, the physio-chemical technique is always more efficient if compared to a single-technique approach regardless of the surface characteristics.
种植体周炎是一种扩散率不断上升的炎症性疾病，会影响修复体长期存留。本研究聚焦于氯己定和碳酸氢钠粉末空气抛光系统对[具体微生物名称未给出]的去污效果，该微生物在病理学中似乎具有超级感染的机会性作用。作者的目的是研究和比较这些临床常用治疗方法的有效性。方法：进行一项体外研究，分析两种广泛用于受影响钛种植体消毒的治疗辅助手段的效果：氯己定（CHX）和碳酸氢钠粉末空气抛光（P）。使用比色测定法（XTT）和扫描电子显微镜（SEM）观察对残留生物膜进行定性和定量比较分析。该实验在机械加工的钛表面和喷砂粗糙表面上进行，目的是找出与种植体表面质地相关的治疗结果差异。原假设是，在进行的处理和钛的纳米结构特征方面，样本之间未检测到差异。当联合使用氯己定和空气抛光（C + P）时，（在两种类型的种植体表面上）均获得了最佳结果。从第一个时间点（30秒）到最后一个时间点（5分钟），在三个不同时间点记录的光密度（OD）值也呈线性下降。在扫描电子显微镜下观察时，粗糙表面显示出广泛且高度结构化的生物膜，与分析机械加工种植体时遇到的生物膜相比更为复杂。结论：本初步研究表明，粗糙表面可促进真菌粘附并最终阻碍去污治疗的效果。为此，无论表面特征如何，物理化学技术相比单一技术方法总是更有效。
