Neumüller J, Tohidast-Akrad M, Ammer K, Hakimzadeh A, Stransky G, Weis S, Partsch G, Eberl R
Ludwig Boltzmann Institute of Rheumatology and Balneology, Vienna-Oberlaa, Austria.
Exp Cell Biol. 1988;56(3):113-30. doi: 10.1159/000163469.
Cell cultures were derived from tendons or ligamentous material from patients with carpal tunnel syndrome (CTS), Dupuytren's contracture (DP), tendopathia nodosa (TN) and hallux valgus (HV). The ultrastructure of the operation specimens as well as of the cell monolayers was investigated, using a floating sheet method in order to preserve both cell-to-cell contacts and the orientation of the monolayers. The histologic features of the tissues obtained in the operations were correlated with the ultrastructure of the cells in culture derived from these specimens. In DP, above all in the nodules, an activation of the capillary endothelium in the vicinity of myofibroblasts and mast cells was observed. In CTS the collagen fibrils varied extremely in diameter. In DP and TN biopsies a splicing process of helicoidly arranged fibrils could be seen. A disintegration of elastic fibers in the fibrillar and amorphous components was found in DP nodules, HV and TN tissues. Transitional forms between fibroblasts and myofibroblasts were observed not only in DP but also-though in a smaller percentage--in the cultures derived from the other patients. The cells showed organelles for active protein synthesis and transport. Autophagocytosis and the formation of multilamellated bodies took place in TN and HV cultures. In CTS, DP and TN cultures cells were connected via gap junctions. In some cultures, above all in those derived from CTS, monocilia were found. In CTS cultures the formation of intracellular collagen occurred. Growth parameters were rather low in HV cultures. PLmax (maximal pulse labelling index) values were higher in TN cultures than in DP and HV cultures. Plating efficiency (PE) values were higher in cultures derived from cell-rich and capillarized tissues than in biopsies with few cells.
细胞培养物来源于患有腕管综合征（CTS）、杜普伊特伦挛缩症（DP）、结节性肌腱病（TN）和拇外翻（HV）患者的肌腱或韧带组织。使用漂浮片法研究手术标本以及细胞单层的超微结构，以保留细胞间接触和单层细胞的取向。将手术中获得的组织的组织学特征与源自这些标本的培养细胞的超微结构进行关联。在DP中，尤其是在结节中，观察到肌成纤维细胞和肥大细胞附近的毛细血管内皮细胞活化。在CTS中，胶原纤维直径差异极大。在DP和TN活检中，可以看到螺旋状排列的纤维的拼接过程。在DP结节、HV和TN组织中发现弹性纤维在纤维状和无定形成分中解体。不仅在DP中，而且在源自其他患者的培养物中（尽管比例较小）也观察到成纤维细胞和肌成纤维细胞之间的过渡形式。细胞显示出用于活跃蛋白质合成和运输的细胞器。在TN和HV培养物中发生自噬和多层体的形成。在CTS、DP和TN培养物中，细胞通过间隙连接相连。在一些培养物中，尤其是源自CTS的培养物中，发现了单纤毛。在CTS培养物中发生细胞内胶原的形成。HV培养物中的生长参数相当低。TN培养物中的PLmax（最大脉冲标记指数）值高于DP和HV培养物。源自富含细胞和有毛细血管化组织的培养物中的接种效率（PE）值高于细胞数量少的活检组织。
