Department of Dermatology, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.
Department of Dermatology, Hebei General Hospital, Shijiazhuang, 050051, Hebei, China.
BMC Complement Med Ther. 2020 Apr 15;20(1):113. doi: 10.1186/s12906-020-02911-w.
To investigate the protective effect of Ixeris Sonchifolia (Bae.) Hance (ISH) extract on herpes simplex virus keratitis (HSK) in mice.
A mouse model of HSK was established by inoculating 60 mice (60 right eyes) with herpes simplex virus type 1 (HSV-1) by corneal scratch. The other 15 mice as blank control only received corneal scratch but without HSV-1. From the 2nd day after the successful modeling, the experimental group was fed with ISH total flavonoids (50, 100 and 200 mg/kg) orally, twice a day for 14 days. The model group and control group were given the same amount of normal saline. The pathological changes of cornea were observed once a day by slit lamp microscopy combined with fluorescein staining. The corneal histopathological examination, the survival status and the serum levels of interleukin-2 (IL-2), IL-4 and interferon-gama (INF-γ) were performed at the end of the experiment.
The result showed that ISH could significantly improve the corneal lesion degree, increase mice survival rate, and markedly increase the levels of IL-2 and INF-γ, reduce the levels of IL-4 in serum of mice.
ISH could increase the anti-virus ability, promote the healing of corneal inflammation and alleviate the pathological damage of cornea, which suggested that ISH has a potential and valuable therapeutic effect on the HSK.
研究了菊三七（Bae.）提取物对单纯疱疹病毒性角膜炎（HSK）小鼠的保护作用。
通过角膜划痕接种 60 只小鼠（60 只右眼）单纯疱疹病毒 1 型（HSV-1）建立 HSK 小鼠模型。另 15 只小鼠作为空白对照，仅接受角膜划痕，而不接受 HSV-1。从成功建模后的第 2 天起，实验组每天两次口服菊三七总黄酮（50、100 和 200mg/kg），连续 14 天。模型组和对照组给予等量的生理盐水。每天用裂隙灯显微镜结合荧光素染色观察一次角膜的病理变化。实验结束时进行角膜组织病理学检查、生存状态以及血清中白细胞介素-2（IL-2）、IL-4 和干扰素-γ（INF-γ）的水平检测。
结果表明，菊三七能显著改善角膜病变程度，提高小鼠存活率，显著增加血清中 IL-2 和 INF-γ的水平，降低 IL-4 的水平。
菊三七能增强抗病毒能力，促进角膜炎症愈合，减轻角膜病理损伤，提示菊三七对 HSK 具有潜在的有价值的治疗作用。
