Zhuhai Precision Medical Center, Zhuhai People's Hospital (Zhuhai Hospital Affiliated With Jinan University), Zhuhai, 519000, Guangdong, P.R. China.
Department of Biochemistry, New York State Center of Excellence in Bioinformatics and Life Sciences, State University of New York at Buffalo, Buffalo, NY, 14203, USA.
Mol Genet Genomics. 2020 Jul;295(4):825-835. doi: 10.1007/s00438-020-01675-9. Epub 2020 Apr 15.
Pioneer transcription factors are a special group of transcription factors that can interact with nucleosomal DNA and initiate regulatory events. Their binding to regulatory regions is the first event in gene activation and can occur in silent or heterochromatin regions. Several research groups have endeavored to define pioneer factors and study their binding characteristics using various techniques. In this review, we describe the in vitro methods used to define and characterize pioneer factors, paying particular attention to differences in methodologies and how these differences can affect results.
先驱转录因子是一类特殊的转录因子，能够与核小体 DNA 相互作用并启动调控事件。它们与调控区域的结合是基因激活的第一步，并且可以发生在沉默或异染色质区域。几个研究小组已经努力定义先驱因子并使用各种技术研究它们的结合特性。在这篇综述中，我们描述了用于定义和表征先驱因子的体外方法，特别关注方法学的差异以及这些差异如何影响结果。
