NMR Spectroscopy Research Group Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht (The, Netherlands.
Chempluschem. 2020 Apr;85(4):760-768. doi: 10.1002/cplu.202000167.
Solution-state NMR spectroscopy has become a powerful tool to study soluble proteins in cells, provided that they tumble sufficiently fast. In addition, cryo-electron tomography (cryo-ET) has recently displayed a tremendous potential to probe structures of large proteins and assemblies in their native cellular environments. However, challenges remain to obtain atomic-level information in native cell settings for proteins that are small, disordered, or are strongly engaged in intermolecular interactions. In this Minireview, we discuss recent progress in using sensitivity enhanced solid-state NMR spectroscopy methods in the context of cellular structural biology.
溶液态 NMR 光谱学已成为研究细胞中可溶性蛋白质的有力工具，前提是它们的旋转速度足够快。此外，冷冻电子断层扫描（cryo-ET）最近显示出探测天然细胞环境中大型蛋白质和复合物结构的巨大潜力。然而，对于小分子、无序或强烈参与分子间相互作用的蛋白质，在天然细胞环境中获得原子水平信息仍然存在挑战。在这篇综述中，我们讨论了在细胞结构生物学背景下使用灵敏度增强的固态 NMR 光谱学方法的最新进展。
