Department of Chemistry, University of California, Irvine 92697-2025, United States; Department of Molecular Biology and Biochemistry, University of California, Irvine 92697-3900, United States.
Department of Chemistry, University of California, Irvine 92697-2025, United States.
J Struct Biol. 2019 Apr 1;206(1):73-89. doi: 10.1016/j.jsb.2018.09.003. Epub 2018 Sep 8.
Many advances in instrumentation and methodology have furthered the use of solid-state NMR as a technique for determining the structures and studying the dynamics of molecules involved in complex biological assemblies. Solid-state NMR does not require large crystals, has no inherent size limit, and with appropriate isotopic labeling schemes, supports solving one component of a complex assembly at a time. It is complementary to cryo-EM, in that it provides local, atomic-level detail that can be modeled into larger-scale structures. This review focuses on the development of high-field MAS instrumentation and methodology; including probe design, benchmarking strategies, labeling schemes, and experiments that enable the use of quadrupolar nuclei in biomolecular NMR. Current challenges facing solid-state NMR of biological assemblies and new directions in this dynamic research area are also discussed.
许多仪器和方法的进步进一步推动了固态 NMR 的应用，使其成为一种用于确定结构和研究参与复杂生物组装的分子动力学的技术。固态 NMR 不需要大晶体，没有内在的尺寸限制，并且通过适当的同位素标记方案，支持一次解决复杂组装体的一个组成部分。它与 cryo-EM 互补，因为它提供了可以建模为更大尺度结构的局部原子级细节。本综述重点介绍了高场 MAS 仪器和方法的发展，包括探头设计、基准测试策略、标记方案以及使生物分子 NMR 能够使用四极核的实验。还讨论了生物组装体的固态 NMR 面临的当前挑战和这一动态研究领域的新方向。
