Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Rd, P. O. Box 398, Beijing, 100083, China.
Key Laboratory of Food Bioengineering, College of Food Science and Nutritional Engineering, (China National Light Industry), China Agricultural University, 17 Qinghua East Rd, P.O. Box 398, Beijing, 100083, China.
World J Microbiol Biotechnol. 2022 Jun 14;38(8):136. doi: 10.1007/s11274-022-03317-1.
Biofilm cells exhibit higher resistance than their planktonic counterparts to commonly used disinfectants in food industry. Phenolic acids are promising substitute offering less selective pressure than traditional antibiotics. This study aims to evaluate the inhibitory effects of ferulic acid (FA) and p-coumaric acid (p-CA) on Salmonella Enteritidis biofilm formation and explore the underlying inhibitory mechanisms. The minimal inhibitory concentration (MIC) of FA and p-CA were 1.0 and 0.5 mg/ml, respectively. The sub-inhibitory concentration (1/8 MIC) significantly decreased biofilm formation without growth inhibitory effects. The biomass and extracellular polymeric substances (EPS) of S. Enteritidis biofilm as well as the bacterial swimming and chemotaxis abilities were significantly decreased when exposed to sub-MIC concentrations of FA and p-CA. These two phenolic acids showed high affinity to proteins involved in flagella motility and repressed the S. Enteritidis biofilm formation-related gene expressions. Furthermore, these two phenolic acids maintained high antibiofilm efficiency in simulated food processing conditions. This study provided valuable information of multiple phenotypic and molecular responses of S. Enteritidis to these two phenolic acids.
生物膜细胞比浮游细胞对食品工业中常用的消毒剂具有更高的抗性。酚酸是一种很有前途的替代品，其选择性压力低于传统抗生素。本研究旨在评估阿魏酸（FA）和对香豆酸（p-CA）对肠炎沙门氏菌生物膜形成的抑制作用，并探讨其潜在的抑制机制。FA 和 p-CA 的最小抑菌浓度（MIC）分别为 1.0 和 0.5mg/ml。亚抑菌浓度（1/8 MIC）显著降低生物膜形成，而无生长抑制作用。当 FA 和 p-CA 的亚 MIC 浓度暴露于生物膜时，肠炎沙门氏菌生物膜的生物量和胞外聚合物（EPS）以及细菌游动和趋化能力均显著降低。这两种酚酸对参与鞭毛运动的蛋白质具有高亲和力，并抑制了肠炎沙门氏菌生物膜形成相关基因的表达。此外，这两种酚酸在模拟食品加工条件下仍保持较高的抗生物膜效率。本研究提供了肠炎沙门氏菌对这两种酚酸的多种表型和分子反应的有价值信息。
