Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, P. R. China.
School of Electronic Science and Engineering, Hunan University of Information Technology, Changsha, 410151, China.
Mikrochim Acta. 2024 Aug 21;191(9):550. doi: 10.1007/s00604-024-06610-y.
A novel bacteriophage-targeted electrochemical biosensor designed for accurate and quantitative detection of live Salmonella in food samples is presented. The biosensor is simply constructed by electrostatic immobilizing bacteriophages on MXene-nanostructured electrodes. MXene, renowned for its high surface area, biocompatibility, and conductivity, serves as an ideal platform for bacteriophage immobilization. This allows for a high-density immobilization of bacteriophage particles, achieving approximately 71 pcs μm. Remarkably, the bacteriophages immobilized MXene nanostructured electrodes still maintain their viability and functionality, ensuring their effectiveness in pathogen detection. Therefore, the proposed biosensor exhibited enhanced sensitivity with a low limit of detection (LOD) of 5 CFU mL. Notably, the biosensor shows excellent specificity in the presence of other bacteria that commonly contaminate food and can distinguish live Salmonella from a mixed population. Furthermore, it is applicable in detecting live Salmonella in food samples, which highlights its potential in food safety monitoring. This biosensor offers simplicity, convenience, and suitability for resource-limited environments, making it a promising tool for on-site monitoring of foodborne pathogenic bacteria.
本文提出了一种新型的细菌噬菌体靶向电化学生物传感器，用于准确、定量地检测食品样品中的活沙门氏菌。该生物传感器通过静电固定在 MXene 纳米结构电极上的噬菌体来简单构建。MXene 因其高比表面积、生物相容性和导电性而闻名，是噬菌体固定的理想平台。这允许噬菌体颗粒的高密度固定，达到约 71 个 pcsm。值得注意的是，固定在 MXene 纳米结构电极上的噬菌体仍然保持其活力和功能，确保了它们在病原体检测中的有效性。因此，所提出的生物传感器具有增强的灵敏度，检测限（LOD）低至 5 CFU mL。值得注意的是，该生物传感器在存在通常污染食物的其他细菌时表现出优异的特异性，并且能够区分混合种群中的活沙门氏菌。此外，它可用于检测食品样品中的活沙门氏菌，突出了其在食品安全监测中的应用潜力。该生物传感器具有简单、方便和适用于资源有限环境的特点，是现场监测食源性病原体的有前途的工具。
