维生素E可改善饲喂含陈化玉米日粮蛋鸡的抗氧化状态，但对其脂质代谢无影响。
Vitamin E improves antioxidant status but not lipid metabolism in laying hens fed a aged corn-containing diet.
作者信息
Ding X M, Mu Y D, Zhang K Y, Wang J P, Bai S P, Zeng Q F, Peng H W
机构信息
Institute of Animal Nutrition, Sichuan Agricultural University, Key laboratory of Animal Disease-resistant Nutrition, Ministry of Education, Key laboratory of Animal Disease-resistant Nutrition, Sichuan Province Chengdu, 611130, China.
出版信息
Anim Biosci. 2021 Feb;34(2):276-284. doi: 10.5713/ajas.19.0934. Epub 2020 Apr 12.
OBJECTIVE
The objective of this study was to determine whether a dietary vitamin E (VE) supplement could alleviate any detrimental effects of aged corn on lipid metabolism and antioxidant status in laying hens.
METHODS
The experiment consisted of a 2×3 factorial design with two corn types (normal corn and aged corn (stored for 4 yr) and three concentrations of VE (0, 20, and 100 IU/kg). A total of 216 Lohmann laying hens (50 wk of age) were randomly allocated into six treatment diets for 12 wk. Each treatment had 6 replicates of 6 hens per replicate.
RESULTS
The results show that aged corn significantly decreased the content of low-density lipoprotein cholesterol (p<0.05), and reduced chemokine-like receptor 1 (CMKLR1) mRNA expression (p<0.05) in the liver compared to controls. Diet with VE did not alter the content of crude fat and cholesterol (p>0.05), or acetyl-CoA carboxylase, lipoprotein lipase, fatty acid synthase or CMKLR1 mRNA expression (p>0.05) in the liver among treatment groups. Aged corn significantly increased the content of malondialdehyde (MDA) (p<0.05) and decreased superoxide dismutase (SOD) activity (p<0.05) in the liver. The VE increased the content of MDA (p<0.05) but decreased glutathione peroxidase (GSH-Px) activity in serum (p<0.01) and in the ovaries (p<0.05). Adding VE at 20 and 100 IU/kg significantly increased GSH-Px activity (p<0.05) in liver and in serum (p<0.01), 100 IU/kg VE significantly increased SOD activity (p<0.05) in serum. Aged corn had no significant effects on GSH-Px mRNA or SOD mRNA expression (p<0.01) in the liver and ovaries. Addition of 100 IU/kg VE could significantly increase SOD mRNA expression (p<0.01) in the liver and ovary.
CONCLUSION
Aged corn affected lipid metabolism and decreased the antioxidant function of laying hens. Dietary VE supplementation was unable to counteract the negative effects of aged corn on lipid metabolism. However, addition of 100 IU/kg VE prevented aged corninduced lipid peroxidation in the organs of laying hens.
目的
本研究的目的是确定膳食补充维生素E（VE）是否可以减轻陈化玉米对蛋鸡脂质代谢和抗氧化状态的任何有害影响。
方法
试验采用2×3析因设计，有两种玉米类型（普通玉米和陈化玉米（储存4年））以及三种VE浓度（0、20和100 IU/kg）。总共216只罗曼蛋鸡（50周龄）被随机分配到六种处理日粮中，为期12周。每个处理有6个重复，每个重复6只母鸡。
结果
结果表明，与对照组相比，陈化玉米显著降低了低密度脂蛋白胆固醇含量（p<0.05），并降低了肝脏中趋化因子样受体1（CMKLR1）mRNA表达（p<0.05）。日粮添加VE并未改变各处理组肝脏中粗脂肪和胆固醇含量（p>0.05），或乙酰辅酶A羧化酶、脂蛋白脂肪酶、脂肪酸合酶或CMKLR1 mRNA表达（p>0.05）。陈化玉米显著增加了肝脏中丙二醛（MDA）含量（p<0.05）并降低了超氧化物歧化酶（SOD）活性（p<0.05）。VE增加了血清（p<0.05）和卵巢（p<0.05）中MDA含量，但降低了血清（p<0.01）和卵巢中谷胱甘肽过氧化物酶（GSH-Px）活性。添加20和100 IU/kg VE显著增加了肝脏和血清中GSH-Px活性（p<0.05），100 IU/kg VE显著增加了血清中SOD活性（p<0.05）。陈化玉米对肝脏和卵巢中GSH-Px mRNA或SOD mRNA表达无显著影响（p<0.01）。添加100 IU/kg VE可显著增加肝脏和卵巢中SOD mRNA表达（p<0.01）。
结论
陈化玉米影响蛋鸡脂质代谢并降低其抗氧化功能。日粮补充VE无法抵消陈化玉米对脂质代谢的负面影响。然而，添加100 IU/kg VE可防止陈化玉米诱导蛋鸡器官脂质过氧化。
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