Department of Chemistry, College of Science, Jouf University, Sakaka 2014, Saudi Arabia; Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Universities and Research Institutes District, Alexandria 21934, Egypt.
Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Universities and Research Institutes District, Alexandria 21934, Egypt; Department of Medicine and Translational Research, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.
Carbohydr Polym. 2020 Jun 15;238:116175. doi: 10.1016/j.carbpol.2020.116175. Epub 2020 Apr 9.
The current study is pertaining to develop a novel wound dressing, comprising natural biologically absorbable materials for wound healing In-vivo. Wound dressing is composed of Polygalacturonic acid, Hyaluronic acid embedded silver nanoparticles, which is further fabricated to form nanofibrous mat, using electrospinning. Silver nanoparticles was prepared using PGA. AgNPs in this formula will serve as an antioxidant and anti-inflammatory that protect cells from destructive effect of elevated ROS and accelerate wound healing. The physical performance and water contact angle for nanofiber was evaluated. The produced nanofiber was characterized by Fourier-transform infrared (FTIR), scanning electron microscopy and thermal analysis. Also, the embedded AgNPs was also characterized by UV-vis spectroscopy and TEM. The nanofiber mates embedded AgNPs was applied to the wounded site of albino rats in-vivo. Histopathological assessment for the wound was fully performed. Also, the antimicrobial activity for the fabricated wound dressing was evaluated against gram+ve and gram -ve bacteria.
本研究旨在开发一种新型的伤口敷料，由天然生物可吸收材料组成，用于体内伤口愈合。伤口敷料由聚半乳糖醛酸、透明质酸嵌入的银纳米粒子组成，进一步通过静电纺丝制成纳米纤维垫。银纳米粒子是使用 PGA 制备的。该配方中的 AgNPs 将作为抗氧化剂和抗炎剂，保护细胞免受升高的 ROS 的破坏作用，并加速伤口愈合。对纳米纤维的物理性能和水接触角进行了评估。通过傅里叶变换红外（FTIR）、扫描电子显微镜和热分析对所制备的纳米纤维进行了表征。此外，还通过紫外可见光谱和 TEM 对嵌入的 AgNPs 进行了表征。将嵌入 AgNPs 的纳米纤维应用于体内白化大鼠的创伤部位。对伤口进行了全面的组织病理学评估。此外，还评估了所制备的伤口敷料对革兰氏阳性菌和革兰氏阴性菌的抗菌活性。
