School of Dentistry, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
School of Dentistry, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
Dent Mater. 2020 Jun;36(6):733-743. doi: 10.1016/j.dental.2020.03.003. Epub 2020 Apr 13.
Current instruments cannot clean in between dental implant threads and effectively remove biofilm from the rough implant surface without damaging it. Cavitation bubbles have the potential to disrupt biofilms. The aim of this study was to see how biofilms can be disrupted using non-contact cavitation from an ultrasonic scaler, imaged inside a restricted implant pocket model using high speed imaging.
Streptococcus sanguinis biofilm was grown for 7 days on dental implants. The implants were placed inside a custom made restricted pocket model and immersed inside a water tank. An ultrasonic scaler tip was placed 0.5mm away from the implant surface and operated at medium power or high power for 2s. The biofilm removal process was imaged using a high speed camera operating at 500 fps. Image analysis was used to calculate the amount of biofilm removed from the high speed images. Scanning electron microscopy was done to visualize the implant surface after cleaning.
Cavitation was able to remove biofilm from dental implants. More biofilm was removed at high power. Scanning electron microscopy showed that the implant surface was clean at the points where the cavitation was most intense. High speed imaging showed biofilm removal underneath implant threads, in areas next to the ultrasonic scaler tip.
A high speed imaging protocol has been developed to visualize and quantify biofilm removal from dental implants in vitro. Cavitation bubbles from dental ultrasonic scalers are able to successfully disrupt biofilm in between implant threads.
目前的器械无法清洁种植牙螺纹之间的部位，也无法有效清除粗糙种植体表面的生物膜而不造成损伤。空化气泡有破坏生物膜的潜力。本研究旨在观察非接触式空化如何利用超声波洁牙器的非接触式空化来破坏生物膜，方法是在受限的种植袋模型内使用高速成像对其进行成像。
在种植牙上培养了 7 天的血链球菌生物膜。将种植体放置在定制的受限口袋模型中，并浸入水箱中。将超声波洁牙器尖端放置在距种植体表面 0.5mm 处，以中功率或高功率运行 2s。使用以 500fps 运行的高速摄像机对生物膜去除过程进行成像。图像分析用于计算从高速图像中去除的生物膜量。清洁后进行扫描电子显微镜检查以观察种植体表面。
空化能够从种植牙上去除生物膜。高功率时去除的生物膜更多。扫描电子显微镜显示，在空化最强烈的地方，种植体表面是干净的。高速成像显示在种植体螺纹下方和超声波洁牙器尖端旁边的区域去除了生物膜。
已经开发出一种高速成像方案，以可视化和量化体外种植牙上的生物膜去除。牙科超声波洁牙器产生的空化气泡能够成功地破坏种植牙螺纹之间的生物膜。
