Obesity and Metabolism Research Unit, USDA, ARS, Western Human Nutrition Research Center, Davis, California, USA.
Department of Nutrition, University of California Davis, Davis, California, USA.
Sci Rep. 2020 Apr 16;10(1):6545. doi: 10.1038/s41598-020-63214-1.
The gut microbiota is a critical mediator of nutrition and disease risk. Like most complex traits, the microbiome is under genetic regulation and differs between inbred strains of mice. We tested the effect of fecal microbiota transplantation (FMT) on obesity, and plasma glucose. For this study, we collected microbiota from 2 inbred strains of mice which differ in adiposity and glucose tolerance, C57BL/6J and WSB/EiJ. C57BL/6J female mice (n = 18) were first treated with antibiotics for 4 weeks to ablate the microbiota. Following ablation, the mice were transplanted with microbiota from a C57BL/6J or a WSB/EiJ mouse and clinical traits and plasma metabolomic profiles were interrogated at 2- and 4-weeks post-transplantation. Unexpectedly, the mice receiving WSB/EiJ microbiota increased adiposity but decreased plasma glucose. Metabolomic and 16S microbiota profiling indicated broad metabolic changes occurred during and after FMT. Detailed analysis of these interactions demonstrated specific microbiota-host metabolite interactions which may alter disease susceptibility.
肠道微生物群是营养和疾病风险的重要介质。与大多数复杂特征一样，微生物组受遗传调控，并在近交系小鼠之间存在差异。我们测试了粪便微生物群移植（FMT）对肥胖和血浆葡萄糖的影响。在这项研究中，我们从两种在肥胖和葡萄糖耐量上存在差异的近交系小鼠（C57BL/6J 和 WSB/EiJ）中收集了微生物群。首先，用抗生素处理 C57BL/6J 雌性小鼠 4 周以消灭微生物群。消灭后，将 C57BL/6J 或 WSB/EiJ 小鼠的微生物群移植到小鼠体内，并在移植后 2 周和 4 周时检测临床特征和血浆代谢组学特征。出乎意料的是，接受 WSB/EiJ 微生物群的小鼠体重增加，但血浆葡萄糖降低。代谢组学和 16S 微生物组分析表明，在 FMT 期间和之后发生了广泛的代谢变化。对这些相互作用的详细分析表明了可能改变疾病易感性的特定微生物群-宿主代谢物相互作用。
