Namdari H, Bottone E J
Department of Microbiology, Mount Sinai Hospital, New York, New York 10029-6574.
J Clin Microbiol. 1988 Dec;26(12):2615-9. doi: 10.1128/jcm.26.12.2615-2619.1988.
Certain strains of mesophilic aeromonads (Aeromonas hydrophila, A. sorbria, and A. caviae), when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. Because these strains are nonviable after 24 h, this phenomenon is termed suicide. We investigated suicidal strains of Aeromonas species as a means of understanding animal virulence and enteropathogenicity. To assess virulence, batches of five white mice were inoculated intraperitoneally with 10(7) cells (washed) of suicidal and nonsuicidal strains of A. hydrophila and A. sobria and suicidal strains of A. caviae. The three nonsuicidal strains of A. sobria tested showed lethality as early as 12 h and were uniformly fatal within 36 h postinoculation. After 36 h, the three suicidal strains killed only 1 of 15 mice inoculated. Four A. hydrophila strains tested which showed the suicide phenomenon at 37 degrees C were variably lethal (40 to 100%). None of three suicidal strains of A. caviae were lethal. Enteropathogenicity was studied by orally inoculating three white mice each with the same Aeromonas strains (10(8) cells, in skim milk) and assessing diarrhea and intestinal fluid accumulation. Diarrhea and fluid accumulation were present in all mice inoculated with two nonsuicidal strains of A. sobria and in 4 of 12 mice given four suicidal strains of A. hydrophila. Two suicidal strains each of A. sorbria and A. caviae failed to elicit any gastrointestinal disturbances. These data suggest that the suicide phenomenon may explain strain-specific (A. sobria and A. hydrophila) and species-specific (A. caviae) virulence and enteropathogenicity.
某些嗜温气单胞菌菌株（嗜水气单胞菌、温和气单胞菌和豚鼠气单胞菌）在含有0.5%葡萄糖的肉汤中生长时，会出现生长抑制并伴随乙酸盐积累。由于这些菌株在24小时后无法存活，这种现象被称为自杀。我们研究了气单胞菌属的自杀菌株，以此作为理解动物毒力和肠道致病性的一种手段。为了评估毒力，将五组每组五只的白色小鼠腹腔注射10⁷个（洗涤过的）嗜水气单胞菌和温和气单胞菌的自杀和非自杀菌株以及豚鼠气单胞菌的自杀菌株。所测试的三株温和气单胞菌非自杀菌株最早在12小时就显示出致死性，接种后36小时内全部致死。36小时后，三株自杀菌株仅杀死了接种的15只小鼠中的1只。所测试的四株嗜水气单胞菌菌株在37℃时表现出自杀现象，其致死率各不相同（40%至100%）。三株豚鼠气单胞菌自杀菌株均无致死性。通过给每组三只白色小鼠口服相同的气单胞菌菌株（10⁸个细胞，在脱脂乳中）并评估腹泻和肠液积聚来研究肠道致病性。接种两株温和气单胞菌非自杀菌株的所有小鼠以及接种四株嗜水气单胞菌自杀菌株的12只小鼠中的4只出现了腹泻和液体积聚。两株温和气单胞菌和豚鼠气单胞菌自杀菌株均未引起任何胃肠道紊乱。这些数据表明，自杀现象可能解释了菌株特异性（温和气单胞菌和嗜水气单胞菌）和种特异性（豚鼠气单胞菌）的毒力和肠道致病性。
